Title | Optogenetic control of protein binding using light-switchable nanobodies. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Gil, AA, Carrasco-López, C, Zhu, L, Zhao, EM, Ravindran, PT, Wilson, MZ, Goglia, AG, Avalos, JL, Toettcher, JE |
Journal | Nat Commun |
Volume | 11 |
Issue | 1 |
Pagination | 4044 |
Date Published | 2020 08 13 |
ISSN | 2041-1723 |
Keywords | Animals, HEK293 Cells, Humans, Mice, NIH 3T3 Cells, Optogenetics, Protein Binding, Protein Transport, Signal Transduction, Synthetic Biology |
Abstract | <p>A growing number of optogenetic tools have been developed to reversibly control binding between two engineered protein domains. In contrast, relatively few tools confer light-switchable binding to a generic target protein of interest. Such a capability would offer substantial advantages, enabling photoswitchable binding to endogenous target proteins in cells or light-based protein purification in vitro. Here, we report the development of opto-nanobodies (OptoNBs), a versatile class of chimeric photoswitchable proteins whose binding to proteins of interest can be enhanced or inhibited upon blue light illumination. We find that OptoNBs are suitable for a range of applications including reversibly binding to endogenous intracellular targets, modulating signaling pathway activity, and controlling binding to purified protein targets in vitro. This work represents a step towards programmable photoswitchable regulation of a wide variety of target proteins.</p> |
DOI | 10.1038/s41467-020-17836-8 |
Alternate Journal | Nat Commun |
PubMed ID | 32792536 |
PubMed Central ID | PMC7426870 |
Grant List | DP2 EB024247 / EB / NIBIB NIH HHS / United States F30 CA206408 / CA / NCI NIH HHS / United States F32 GM128304 / GM / NIGMS NIH HHS / United States |