Optogenetic Control of Microbial Consortia Populations for Chemical Production.

TitleOptogenetic Control of Microbial Consortia Populations for Chemical Production.
Publication TypeJournal Article
Year of Publication2021
AuthorsLalwani, MA, Kawabe, H, Mays, RL, Hoffman, SM, Avalos, JL
JournalACS Synth Biol
Date Published2021 08 20
KeywordsBiosynthetic Pathways, Coculture Techniques, Escherichia coli, Metabolic Engineering, Microbial Consortia, Optogenetics, Saccharomyces cerevisiae

<p>Microbial co-culture fermentations can improve chemical production from complex biosynthetic pathways over monocultures by distributing enzymes across multiple strains, thereby reducing metabolic burden, overcoming endogenous regulatory mechanisms, or exploiting natural traits of different microbial species. However, stabilizing and optimizing microbial subpopulations for maximal chemical production remains a major obstacle in the field. In this study, we demonstrate that optogenetics is an effective strategy to dynamically control populations in microbial co-cultures. Using a new optogenetic circuit we call OptoTA, we regulate an endogenous toxin-antitoxin system, enabling tunability of growth using only blue light. With this system we can control the population composition of co-cultures of and . When introducing in each strain different metabolic modules of biosynthetic pathways for isobutyl acetate or naringenin, we found that the productivity of co-cultures increases by adjusting the population ratios with specific light duty cycles. This study shows the feasibility of using optogenetics to control microbial consortia populations and the advantages of using light to control their chemical production.</p>

Alternate JournalACS Synth Biol
PubMed ID34351122