Nucleic Acid-Barcoding Technologies: Converting DNA Sequencing into a Broad-Spectrum Molecular Counter. Author Glen Liszczak, Tom Muir Publication Year 2019 Type Journal Article Abstract The emergence of high-throughput DNA sequencing technologies sparked a revolution in the field of genomics that has rippled into many branches of the life and physical sciences. The remarkable sensitivity, specificity, throughput, and multiplexing capacity that are inherent to parallel DNA sequencing have since motivated its use as a broad-spectrum molecular counter. A key aspect of extrapolating DNA sequencing to non-traditional applications is the need to append nucleic-acid barcodes to entities of interest. In this review, we describe the chemical and biochemical approaches that have enabled nucleic-acid barcoding of proteinaceous and non-proteinaceous materials and provide examples of downstream technologies that have been made possible by DNA-encoded molecules. As commercially available high-throughput sequencers were first released less than 15 years ago, we believe related applications will continue to mature and close by proposing new frontiers to support this assertion. Keywords Animals, Humans, Computational Biology, Proteins, High-Throughput Nucleotide Sequencing, Nucleic Acids, DNA Barcoding, Taxonomic Journal Angew Chem Int Ed Engl Volume 58 Issue 13 Pages 4144-4162 Date Published 2019 Mar 22 ISSN Number 1521-3773 DOI 10.1002/anie.201808956 Alternate Journal Angew Chem Int Ed Engl PMCID PMC6748390 PMID 30153374 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML