Normal human cell proteins that interact with the adenovirus type 5 E1B 55kDa protein.

TitleNormal human cell proteins that interact with the adenovirus type 5 E1B 55kDa protein.
Publication TypeJournal Article
Year of Publication2017
AuthorsHung, G, Flint, SJ
Date Published2017 Apr
KeywordsA549 Cells, Adenovirus E1B Proteins, Adenovirus E4 Proteins, Adenoviruses, Human, Amino Acid Sequence, DNA, Viral, Genome, Viral, HEK293 Cells, HeLa Cells, Humans, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, Repressor Proteins, RNA Interference, RNA, Small Interfering, RNA-Binding Proteins, Sin3 Histone Deacetylase and Corepressor Complex, Virus Replication

<p>Several of the functions of the human adenovirus type 5 E1B 55kDa protein are fulfilled via the virus-specific E3 ubiquitin ligase it forms with the viral E4 Orf6 protein and several cellular proteins. Important substrates of this enzyme have not been identified, and other functions, including repression of transcription of interferon-sensitive genes, do not require the ligase. We therefore used immunoaffinity purification and liquid chromatography-mass spectrometry of lysates of normal human cells infected in parallel with HAdV-C5 and E1B 55kDa protein-null mutant viruses to identify specifically E1B 55kDa-associated proteins. The resulting set of >90 E1B-associated proteins contained the great majority identified previously, and was enriched for those associated with the ubiquitin-proteasome system, RNA metabolism and the cell cycle. We also report very severe inhibition of viral genome replication when cells were exposed to both specific or non-specific siRNAs and interferon prior to infection.</p>

Alternate JournalVirology
PubMed ID28135605
PubMed Central IDPMC5337154
Grant ListR56 AI091785 / AI / NIAID NIH HHS / United States