New mouse models for high resolution and live imaging of planar cell polarity proteins in vivo.

TitleNew mouse models for high resolution and live imaging of planar cell polarity proteins in vivo.
Publication TypeJournal Article
Year of Publication2021
AuthorsBasta, LP, Hill-Oliva, M, Paramore, SV, Sharan, R, Goh, A, Biswas, A, Cortez, M, Little, KA, Posfai, E, Devenport, D
JournalDevelopment
Volume148
Issue18
Date Published2021 09 15
ISSN1477-9129
KeywordsAnimals, Body Patterning, Cell Polarity, Diagnostic Imaging, Embryo, Mammalian, Epidermal Cells, Epidermis, Epithelium, Frizzled Receptors, Membrane Proteins, Mice, Mice, Inbred C57BL, Models, Animal, Nerve Tissue Proteins, Neural Tube, Receptors, G-Protein-Coupled, Signal Transduction, Trachea
Abstract

<p>The collective polarization of cellular structures and behaviors across a tissue plane is a near universal feature of epithelia known as planar cell polarity (PCP). This property is controlled by the core PCP pathway, which consists of highly conserved membrane-associated protein complexes that localize asymmetrically at cell junctions. Here, we introduce three new mouse models for investigating the localization and dynamics of transmembrane PCP proteins: Celsr1, Fz6 and Vangl2. Using the skin epidermis as a model, we characterize and verify the expression, localization and function of endogenously tagged Celsr1-3xGFP, Fz6-3xGFP and tdTomato-Vangl2 fusion proteins. Live imaging of Fz6-3xGFP in basal epidermal progenitors reveals that the polarity of the tissue is not fixed through time. Rather, asymmetry dynamically shifts during cell rearrangements and divisions, while global, average polarity of the tissue is preserved. We show using super-resolution STED imaging that Fz6-3xGFP and tdTomato-Vangl2 can be resolved, enabling us to observe their complex localization along junctions. We further explore PCP fusion protein localization in the trachea and neural tube, and discover new patterns of PCP expression and localization throughout the mouse embryo.</p>

DOI10.1242/dev.199695
Alternate JournalDevelopment
PubMed ID34463728
PubMed Central IDPMC8487645
Grant ListR01 AR066070 / AR / NIAMS NIH HHS / United States
R01 AR068320 / AR / NIAMS NIH HHS / United States
F31 AR077407 / AR / NIAMS NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States