New developments in RiPP discovery, enzymology and engineering.

TitleNew developments in RiPP discovery, enzymology and engineering.
Publication TypeJournal Article
Year of Publication2021
AuthorsMontalbán-López, M, Scott, TA, Ramesh, S, Rahman, IR, van Heel, AJ, Viel, JH, Bandarian, V, Dittmann, E, Genilloud, O, Goto, Y, Burgos, MJosé Gran, Hill, C, Kim, S, Koehnke, J, Latham, JA, A Link, J, Martínez, B, Nair, SK, Nicolet, Y, Rebuffat, S, Sahl, H-G, Sareen, D, Schmidt, EW, Schmitt, L, Severinov, K, Süssmuth, RD, Truman, AW, Wang, H, Weng, J-K, van Wezel, GP, Zhang, Q, Zhong, J, Piel, J, Mitchell, DA, Kuipers, OP, van der Donk, WA
JournalNat Prod Rep
Date Published2021 Jan 01
KeywordsBiological Products, Computational Biology, Enzymes, Hydroxylation, Methylation, Peptides, Phosphorylation, Protein Engineering, Protein Processing, Post-Translational, Protein Sorting Signals, Ribosomes

<p>Covering: up to June 2020Ribosomally-synthesized and post-translationally modified peptides (RiPPs) are a large group of natural products. A community-driven review in 2013 described the emerging commonalities in the biosynthesis of RiPPs and the opportunities they offered for bioengineering and genome mining. Since then, the field has seen tremendous advances in understanding of the mechanisms by which nature assembles these compounds, in engineering their biosynthetic machinery for a wide range of applications, and in the discovery of entirely new RiPP families using bioinformatic tools developed specifically for this compound class. The First International Conference on RiPPs was held in 2019, and the meeting participants assembled the current review describing new developments since 2013. The review discusses the new classes of RiPPs that have been discovered, the advances in our understanding of the installation of both primary and secondary post-translational modifications, and the mechanisms by which the enzymes recognize the leader peptides in their substrates. In addition, genome mining tools used for RiPP discovery are discussed as well as various strategies for RiPP engineering. An outlook section presents directions for future research.</p>

Alternate JournalNat Prod Rep
PubMed ID32935693
PubMed Central IDPMC7864896
Grant ListR37 GM058822 / GM / NIGMS NIH HHS / United States
/ HHMI / Howard Hughes Medical Institute / United States
BBS/E/J/000PR9790 / BB_ / Biotechnology and Biological Sciences Research Council / United Kingdom
R01 GM123998 / GM / NIGMS NIH HHS / United States
R01 AI144967 / AI / NIAID NIH HHS / United States
R01 GM131347 / GM / NIGMS NIH HHS / United States
T32 GM070421 / GM / NIGMS NIH HHS / United States
R01 GM079038 / GM / NIGMS NIH HHS / United States
R01 AI117210 / AI / NIAID NIH HHS / United States