Neuronal identities derived by misexpression of the POU IV sensory determinant in a protovertebrate.

TitleNeuronal identities derived by misexpression of the POU IV sensory determinant in a protovertebrate.
Publication TypeJournal Article
Year of Publication2022
AuthorsChacha, PPaul, Horie, R, Kusakabe, TG, Sasakura, Y, Singh, M, Horie, T, Levine, M
JournalProc Natl Acad Sci U S A
Volume119
Issue4
Date Published2022 01 25
ISSN1091-6490
KeywordsAnimals, Biological Evolution, Cellular Reprogramming, Ciona intestinalis, Epidermis, Gene Expression, Gene Expression Regulation, Developmental, Gene Regulatory Networks, Neural Crest, Neural Plate, Neurons, POU Domain Factors, Single-Cell Analysis, Transcription Factors, Vertebrates
Abstract

<p>The protovertebrate type A (sometimes called ) contains a series of sensory cell types distributed across the head-tail axis of swimming tadpoles. They arise from lateral regions of the neural plate that exhibit properties of vertebrate placodes and neural crest. The sensory determinant is known to work in concert with regional determinants, such as and , to produce palp sensory cells (PSCs) and bipolar tail neurons (BTNs), in head and tail regions, respectively. A combination of single-cell RNA-sequencing (scRNA-seq) assays, computational analysis, and experimental manipulations suggests that misexpression of results in variable transformations of epidermal cells into hybrid sensory cell types, including those exhibiting properties of both PSCs and BTNs. Hybrid properties are due to coexpression of and that is triggered by an unexpected feedback loop. Hybrid cells were also found to express a synthetic gene battery that is not coexpressed in any known cell type. We discuss these results with respect to the opportunities and challenges of reprogramming cell types through the targeted misexpression of cellular determinants.</p>

DOI10.1073/pnas.2118817119
Alternate JournalProc Natl Acad Sci U S A
PubMed ID35042818
PubMed Central IDPMC8794889
Grant ListR01 NS076542 / NS / NINDS NIH HHS / United States
T32 HG003284 / HG / NHGRI NIH HHS / United States