Near-equilibrium glycolysis supports metabolic homeostasis and energy yield.

TitleNear-equilibrium glycolysis supports metabolic homeostasis and energy yield.
Publication TypeJournal Article
Year of Publication2019
AuthorsPark, JO, Tanner, LB, Wei, MH, Khana, DB, Jacobson, TB, Zhang, Z, Rubin, SA, Li, SHsin-Jung, Higgins, MB, Stevenson, DM, Amador-Noguez, D, Rabinowitz, JD
JournalNat Chem Biol
Volume15
Issue10
Pagination1001-1008
Date Published2019 Oct
ISSN1552-4469
Abstract

Glycolysis plays a central role in producing ATP and biomass. Its control principles, however, remain incompletely understood. Here, we develop a method that combines 2H and 13C tracers to determine glycolytic thermodynamics. Using this method, we show that, in conditions and organisms with relatively slow fluxes, multiple steps in glycolysis are near to equilibrium, reflecting spare enzyme capacity. In Escherichia coli, nitrogen or phosphorus upshift rapidly increases the thermodynamic driving force, deploying the spare enzyme capacity to increase flux. Similarly, respiration inhibition in mammalian cells rapidly increases both glycolytic flux and the thermodynamic driving force. The thermodynamic shift allows flux to increase with only small metabolite concentration changes. Finally, we find that the cellulose-degrading anaerobe Clostridium cellulolyticum exhibits slow, near-equilibrium glycolysis due to the use of pyrophosphate rather than ATP for fructose-bisphosphate production, resulting in enhanced per-glucose ATP yield. Thus, near-equilibrium steps of glycolysis promote both rapid flux adaptation and energy efficiency.

DOI10.1038/s41589-019-0364-9
Alternate JournalNat. Chem. Biol.
PubMed ID31548693
Grant ListDE-AC05-00OR22725 / / U.S. Department of Energy (DOE) /
DE-SC0012461 / / U.S. Department of Energy (DOE) /
DE-SC0018420 / / U.S. Department of Energy (DOE) /