Multiple signalling systems controlling expression of luminescence in Vibrio harveyi: sequence and function of genes encoding a second sensory pathway.

TitleMultiple signalling systems controlling expression of luminescence in Vibrio harveyi: sequence and function of genes encoding a second sensory pathway.
Publication TypeJournal Article
Year of Publication1994
AuthorsBassler, BL, Wright, M, Silverman, MR
JournalMol Microbiol
Volume13
Issue2
Pagination273-86
Date Published1994 Jul
ISSN0950-382X
Keywords4-Butyrolactone, Amino Acid Sequence, Bacterial Proteins, Base Sequence, Gene Expression Regulation, Bacterial, Luminescent Measurements, Models, Biological, Molecular Sequence Data, Mutagenesis, Insertional, Phenotype, Phosphorylation, Phosphotransferases, Protein Kinases, Protein Processing, Post-Translational, Repressor Proteins, Sequence Alignment, Sequence Homology, Amino Acid, Signal Transduction, Trans-Activators, Transcription Factors, Vibrio
Abstract

<p>Density-dependent expression of luminescence in Vibrio harveyi is regulated by the concentration of extracellular signal molecules (autoinducers) in the culture medium. One signal-response system is encoded by the luxL,M,N locus. The luxL and luxM genes are required for the production of an autoinducer (probably beta-hydroxybutyl homoserine lactone), and the luxN gene is required for the response to that autoinducer. Analysis of the phenotypes of LuxL,M and N mutants indicated that an additional signal-response system also controls density sensing. We report here the identification, cloning and analysis of luxP and luxQ, which encode functions required for a second density-sensing system. Mutants with defects in luxP and luxQ are defective in response to a second autoinducer substance. LuxQ, like LuxN, is similar to members of the family of two-component, signal transduction proteins and contains both a histidine protein kinase and a response regulator domain. Analysis of signalling mutant phenotypes indicates that there are at least two separate signal-response pathways which converge to regulate expression of luminescence in V. harveyi.</p>

DOI10.1111/j.1365-2958.1994.tb00422.x
Alternate JournalMol. Microbiol.
PubMed ID7984107