Title | Multiple signalling systems controlling expression of luminescence in Vibrio harveyi: sequence and function of genes encoding a second sensory pathway. |
Publication Type | Journal Article |
Year of Publication | 1994 |
Authors | Bassler, BL, Wright, M, Silverman, MR |
Journal | Mol Microbiol |
Volume | 13 |
Issue | 2 |
Pagination | 273-86 |
Date Published | 1994 Jul |
ISSN | 0950-382X |
Keywords | 4-Butyrolactone, Amino Acid Sequence, Bacterial Proteins, Base Sequence, Gene Expression Regulation, Bacterial, Luminescent Measurements, Models, Biological, Molecular Sequence Data, Mutagenesis, Insertional, Phenotype, Phosphorylation, Phosphotransferases, Protein Kinases, Protein Processing, Post-Translational, Repressor Proteins, Sequence Alignment, Sequence Homology, Amino Acid, Signal Transduction, Trans-Activators, Transcription Factors, Vibrio |
Abstract | <p>Density-dependent expression of luminescence in Vibrio harveyi is regulated by the concentration of extracellular signal molecules (autoinducers) in the culture medium. One signal-response system is encoded by the luxL,M,N locus. The luxL and luxM genes are required for the production of an autoinducer (probably beta-hydroxybutyl homoserine lactone), and the luxN gene is required for the response to that autoinducer. Analysis of the phenotypes of LuxL,M and N mutants indicated that an additional signal-response system also controls density sensing. We report here the identification, cloning and analysis of luxP and luxQ, which encode functions required for a second density-sensing system. Mutants with defects in luxP and luxQ are defective in response to a second autoinducer substance. LuxQ, like LuxN, is similar to members of the family of two-component, signal transduction proteins and contains both a histidine protein kinase and a response regulator domain. Analysis of signalling mutant phenotypes indicates that there are at least two separate signal-response pathways which converge to regulate expression of luminescence in V. harveyi.</p> |
DOI | 10.1111/j.1365-2958.1994.tb00422.x |
Alternate Journal | Mol Microbiol |
PubMed ID | 7984107 |