A multilayered scaffold for regeneration of smooth muscle and connective tissue layers.

TitleA multilayered scaffold for regeneration of smooth muscle and connective tissue layers.
Publication TypeJournal Article
Year of Publication2020
AuthorsGarrison, CM, Singh-Varma, A, Pastino, AK, Steele, JAM, Kohn, J, N Murthy, S, Schwarzbauer, JE
JournalJ Biomed Mater Res A
Date Published2020 Jul 12
ISSN1552-4965
Abstract

<p>Tissue regeneration often requires recruitment of different cell types and rebuilding of two or more tissue layers to restore function. Here, we describe the creation of a novel multilayered scaffold with distinct fiber organizations-aligned to unaligned and dense to porous-to template common architectures found in adjacent tissue layers. Electrospun scaffolds were fabricated using a biodegradable, tyrosine-derived terpolymer, yielding densely-packed, aligned fibers that transition into randomly-oriented fibers of increasing diameter and porosity. We demonstrate that differently-oriented scaffold fibers direct cell and extracellular matrix (ECM) organization, and that scaffold fibers and ECM protein networks are maintained after decellularization. Smooth muscle and connective tissue layers are frequently adjacent in vivo; we show that within a single scaffold, the architecture supports alignment of contractile smooth muscle cells and deposition by fibroblasts of a meshwork of ECM fibrils. We rolled a flat scaffold into a tubular construct and, after culture, showed cell viability, orientation, and tissue-specific protein expression in the tube were similar to the flat-sheet scaffold. This scaffold design not only has translational potential for reparation of flat and tubular tissue layers but can also be customized for alternative applications by introducing two or more cell types in different combinations.</p>

DOI10.1002/jbm.a.37058
Alternate JournalJ Biomed Mater Res A
PubMed ID32654327
Grant ListR01 AR 073236 / AR / NIAMS NIH HHS / United States
P41 EB001046 / EB / NIBIB NIH HHS / United States
P4 EB001046 / EB / NIBIB NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States
NIH T32 GM007388 / GM / NIGMS NIH HHS / United States
R01 AR073236 / AR / NIAMS NIH HHS / United States
DFHS18PPC041 / / New Jersey Commission on Cancer Research (NJCCR) /