Title | mRNA localises to the oocyte anterior by random Dynein-mediated transport and anchoring. |
Publication Type | Journal Article |
Year of Publication | 2016 |
Authors | Trovisco, V, Belaya, K, Nashchekin, D, Irion, U, Sirinakis, G, Butler, R, Lee, JJ, Gavis, ER, St Johnston, D |
Journal | Elife |
Volume | 5 |
Date Published | 2016 Oct 28 |
ISSN | 2050-084X |
Keywords | Animals, Biological Transport, Active, Drosophila, Drosophila Proteins, Dyneins, Homeodomain Proteins, Oocytes, Ribonucleoproteins, RNA, Messenger, Trans-Activators |
Abstract | <p> mRNA localises to the oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Furthermore, mRNA localises normally in , which abolishes the polarised microtubule organisation. FRAP and photo-conversion experiments demonstrate that the RNA is stably anchored at the anterior, independently of microtubules. Thus, mRNA is localised by random active transport and anterior anchoring. Super-resolution imaging reveals that mRNA forms 110-120 nm particles with variable RNA content, but constant size. These particles appear to be well-defined structures that package the RNA for transport and anchoring.</p> |
DOI | 10.7554/eLife.17537 |
Alternate Journal | Elife |
PubMed ID | 27791980 |
PubMed Central ID | PMC5125753 |
Grant List | 092096 / WT_ / Wellcome Trust / United Kingdom R01 GM067758 / GM / NIGMS NIH HHS / United States 092096 / / Wellcome Trust / United Kingdom A14492 / CRUK_ / Cancer Research UK / United Kingdom BB/F010303/1 / BB_ / Biotechnology and Biological Sciences Research Council / United Kingdom 095297 / WT_ / Wellcome Trust / United Kingdom A14492 / CRUK_ / Cancer Research UK / United Kingdom 080007 / WT_ / Wellcome Trust / United Kingdom |