MreB polymers and curvature localization are enhanced by RodZ and predict E. coli's cylindrical uniformity.

TitleMreB polymers and curvature localization are enhanced by RodZ and predict E. coli's cylindrical uniformity.
Publication TypeJournal Article
Year of Publication2018
AuthorsBratton, BP, Shaevitz, JW, Gitai, Z, Morgenstein, RM
JournalNat Commun
Volume9
Issue1
Pagination2797
Date Published2018 07 18
ISSN2041-1723
KeywordsAmdinocillin, Anti-Bacterial Agents, Cell Membrane, Cell Wall, Cytoskeletal Proteins, Escherichia coli, Escherichia coli Proteins, Gene Expression Regulation, Bacterial, Green Fluorescent Proteins, Machine Learning, Protein Multimerization, Recombinant Fusion Proteins, Regression Analysis
Abstract

<p>The actin-like protein MreB has been proposed to coordinate the synthesis of the cell wall to determine cell shape in bacteria. MreB is preferentially localized to areas of the cell with specific curved geometries, avoiding the cell poles. It remains unclear whether MreB's curvature preference is regulated by additional factors, and which specific features of MreB promote specific features of rod shape growth. Here, we show that the transmembrane protein RodZ modulates MreB curvature preference and polymer number in E. coli, properties which are regulated independently. An unbiased machine learning analysis shows that MreB polymer number, the total length of MreB polymers, and MreB curvature preference are key correlates of cylindrical uniformity, the variability in radius within a single cell. Changes in the values of these parameters are highly predictive of the resulting changes in cell shape (r = 0.93). Our data thus suggest RodZ promotes the assembly of geometrically-localized MreB polymers that lead to the growth of uniform cylinders.</p>

DOI10.1038/s41467-018-05186-5
Alternate JournalNat Commun
PubMed ID30022070
PubMed Central IDPMC6052060
Grant ListR21 AI121828 / AI / NIAID NIH HHS / United States
F32 GM103290 / GM / NIGMS NIH HHS / United States
PHY1734030 / / NSF | Directorate for Mathematical &amp; Physical Sciences | Division of Physics (PHY) / International
R01GM107384 / / U.S. Department of Health &amp; Human Services | NIH | National Institute of General Medical Sciences (NIGMS) / International
R21AI121828 / / U.S. Department of Health &amp; Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID) / International
R01 GM107384 / GM / NIGMS NIH HHS / United States
F32GM103290 / / U.S. Department of Health &amp; Human Services | NIH | National Institute of General Medical Sciences (NIGMS) / International