A molecular engineering toolbox for the structural biologist.

TitleA molecular engineering toolbox for the structural biologist.
Publication TypeJournal Article
Year of Publication2017
AuthorsDebelouchina, GT, Muir, TW
JournalQ Rev Biophys
Volume50
Paginatione7
Date Published2017 01
ISSN1469-8994
KeywordsAnimals, Biology, Engineering, Humans, Protein Engineering
Abstract

<p>Exciting new technological developments have pushed the boundaries of structural biology, and have enabled studies of biological macromolecules and assemblies that would have been unthinkable not long ago. Yet, the enhanced capabilities of structural biologists to pry into the complex molecular world have also placed new demands on the abilities of protein engineers to reproduce this complexity into the test tube. With this challenge in mind, we review the contents of the modern molecular engineering toolbox that allow the manipulation of proteins in a site-specific and chemically well-defined fashion. Thus, we cover concepts related to the modification of cysteines and other natural amino acids, native chemical ligation, intein and sortase-based approaches, amber suppression, as well as chemical and enzymatic bio-conjugation strategies. We also describe how these tools can be used to aid methodology development in X-ray crystallography, nuclear magnetic resonance, cryo-electron microscopy and in the studies of dynamic interactions. It is our hope that this monograph will inspire structural biologists and protein engineers alike to apply these tools to novel systems, and to enhance and broaden their scope to meet the outstanding challenges in understanding the molecular basis of cellular processes and disease.</p>

DOI10.1017/S0033583517000051
Alternate JournalQ Rev Biophys
PubMed ID29233219
PubMed Central IDPMC5978726
Grant ListP01 CA196539 / CA / NCI NIH HHS / United States
R01 GM107047 / GM / NIGMS NIH HHS / United States
R37 GM086868 / GM / NIGMS NIH HHS / United States