Type

Journal Article
Abstract

Modern proximity labeling techniques have enabled significant advances in understanding biomolecular interactions. However, current tools primarily utilize activation modes that are incompatible with complex biological environments, limiting our ability to interrogate cell- and tissue-level microenvironments in animal models. Here, we report μMap-Red, a proximity labeling platform that uses a red-light-excited Sn chlorin e6 catalyst to activate a phenyl azide biotin probe. We validate μMap-Red by demonstrating photonically controlled protein labeling through several layers of tissue, and we then apply our platform to label microenvironments and validate performance with STED microscopy and quantitative proteomics. Finally, to demonstrate labeling in a complex biological sample, we deploy μMap-Red in whole mouse blood to profile erythrocyte cell-surface proteins. This work represents a significant methodological advance toward light-based proximity labeling in complex tissue environments and animal models.

Journal
J Am Chem Soc
Volume
144
Issue
14
Pages
6154-6162
Date Published
2022 Apr 13
ISSN Number
1520-5126
Alternate Journal
J Am Chem Soc
PMCID
PMC9843638
PMID
35363468