μMap-Red: Proximity Labeling by Red Light Photocatalysis.

TitleμMap-Red: Proximity Labeling by Red Light Photocatalysis.
Publication TypeJournal Article
Year of Publication2022
AuthorsBuksh, BF, Knutson, SD, Oakley, JV, Bissonnette, NB, Oblinsky, DG, Schwoerer, MP, Seath, CP, Geri, JB, Rodriguez-Rivera, FP, Parker, DL, Scholes, GD, Ploss, A, MacMillan, DWC
JournalJ Am Chem Soc
Volume144
Issue14
Pagination6154-6162
Date Published2022 Apr 13
ISSN1520-5126
KeywordsAnimals, Biotin, Light, Membrane Proteins, Mice, Proteomics, Staining and Labeling
Abstract

<p>Modern proximity labeling techniques have enabled significant advances in understanding biomolecular interactions. However, current tools primarily utilize activation modes that are incompatible with complex biological environments, limiting our ability to interrogate cell- and tissue-level microenvironments in animal models. Here, we report μMap-Red, a proximity labeling platform that uses a red-light-excited Sn chlorin e6 catalyst to activate a phenyl azide biotin probe. We validate μMap-Red by demonstrating photonically controlled protein labeling through several layers of tissue, and we then apply our platform to label microenvironments and validate performance with STED microscopy and quantitative proteomics. Finally, to demonstrate labeling in a complex biological sample, we deploy μMap-Red in whole mouse blood to profile erythrocyte cell-surface proteins. This work represents a significant methodological advance toward light-based proximity labeling in complex tissue environments and animal models.</p>

DOI10.1021/jacs.2c01384
Alternate JournalJ Am Chem Soc
PubMed ID35363468
Grant ListR35 GM134897 / GM / NIGMS NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States
P30 CA072720 / CA / NCI NIH HHS / United States