Mixing and matching siderophore clusters: structure and biosynthesis of serratiochelins from Serratia sp. V4.

TitleMixing and matching siderophore clusters: structure and biosynthesis of serratiochelins from Serratia sp. V4.
Publication TypeJournal Article
Year of Publication2012
AuthorsSeyedsayamdost, MR, Cleto, S, Carr, G, Vlamakis, H, Vieira, MJoão, Kolter, R, Clardy, J
JournalJ Am Chem Soc
Volume134
Issue33
Pagination13550-3
Date Published2012 Aug 22
ISSN1520-5126
KeywordsComputational Biology, Escherichia coli, Genes, Bacterial, Multigene Family, Mutation, Serratia, Siderophores, Vibrio cholerae
Abstract

<p>Interrogation of the evolutionary history underlying the remarkable structures and biological activities of natural products has been complicated by not knowing the functions they have evolved to fulfill. Siderophores-soluble, low molecular weight compounds-have an easily understood and measured function: acquiring iron from the environment. Bacteria engage in a fierce competition to acquire iron, which rewards the production of siderophores that bind iron tightly and cannot be used or pirated by competitors. The structures and biosyntheses of "odd" siderophores can reveal the evolutionary strategy that led to their creation. We report a new Serratia strain that produces serratiochelin and an analog of serratiochelin. A genetic approach located the serratiochelin gene cluster, and targeted mutations in several genes implicated in serratiochelin biosynthesis were generated. Bioinformatic analyses and mutagenesis results demonstrate that genes from two well-known siderophore clusters, the Escherichia coli enterobactin cluster and the Vibrio cholera vibriobactin cluster, were shuffled to produce a new siderophore biosynthetic pathway. These results highlight how modular siderophore gene clusters can be mixed and matched during evolution to generate structural diversity in siderophores.</p>

DOI10.1021/ja304941d
Alternate JournalJ. Am. Chem. Soc.
PubMed ID22830960
PubMed Central IDPMC3424848
Grant ListR01 GM082137 / GM / NIGMS NIH HHS / United States
K99 GM098299 / GM / NIGMS NIH HHS / United States
R01 GM086258 / GM / NIGMS NIH HHS / United States
U54 AI057159 / AI / NIAID NIH HHS / United States
GM82137 / GM / NIGMS NIH HHS / United States
1K99 GM098299-01 / GM / NIGMS NIH HHS / United States
GM086258 / GM / NIGMS NIH HHS / United States
AI057159 / AI / NIAID NIH HHS / United States
R00 GM098299 / GM / NIGMS NIH HHS / United States