Mitotic Control of Planar Cell Polarity by Polo-like Kinase 1. Author Rezma Shrestha, Katherine Little, Joel Tamayo, Wenyang Li, David Perlman, Danelle Devenport Publication Year 2015 Type Journal Article Abstract During cell division, polarized epithelial cells employ mechanisms to preserve cell polarity and tissue integrity. In dividing cells of the mammalian skin, planar cell polarity (PCP) is maintained through the bulk internalization, equal segregation, and polarized recycling of cortical PCP proteins. The dramatic redistribution of PCP proteins coincides precisely with cell-cycle progression, but the mechanisms coordinating PCP and mitosis are unknown. Here we identify Plk1 as a master regulator of PCP dynamics during mitosis. Plk1 interacts with core PCP component Celsr1 via a conserved polo-box domain (PBD)-binding motif, localizes to mitotic endosomes, and directly phosphorylates Celsr1. Plk1-dependent phosphorylation activates the endocytic motif specifically during mitosis, allowing bulk recruitment of Celsr1 into endosomes. Inhibiting Plk1 activity blocks PCP internalization and perturbs PCP asymmetry. Mimicking dileucine motif phosphorylation is sufficient to drive Celsr1 internalization during interphase. Thus, Plk1-mediated phosphorylation of Celsr1 ensures that PCP redistribution is precisely coordinated with mitotic entry. Keywords Animals, Mice, Molecular Sequence Data, Humans, Protein Binding, HeLa Cells, Phosphorylation, Amino Acid Sequence, Sequence Homology, Amino Acid, Fluorescent Antibody Technique, Cell Cycle Proteins, Mitosis, Cell Polarity, Amino Acid Motifs, Endocytosis, Endosomes, Interphase, Keratinocytes, Proto-Oncogene Proteins, Receptors, G-Protein-Coupled, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Protein Serine-Threonine Kinases Journal Dev Cell Volume 33 Issue 5 Pages 522-34 Date Published 2015 Jun 08 ISSN Number 1878-1551 DOI 10.1016/j.devcel.2015.03.024 Alternate Journal Dev Cell PMCID PMC4464975 PMID 26004507 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML