Title | Mitotic Control of Planar Cell Polarity by Polo-like Kinase 1. |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Shrestha, R, Little, KA, Tamayo, JV, Li, W, Perlman, DH, Devenport, D |
Journal | Dev Cell |
Volume | 33 |
Issue | 5 |
Pagination | 522-34 |
Date Published | 2015 Jun 08 |
ISSN | 1878-1551 |
Keywords | Amino Acid Motifs, Amino Acid Sequence, Animals, Cell Cycle Proteins, Cell Polarity, Endocytosis, Endosomes, Fluorescent Antibody Technique, HeLa Cells, Humans, Interphase, Keratinocytes, Mice, Mitosis, Molecular Sequence Data, Phosphorylation, Protein Binding, Protein Serine-Threonine Kinases, Proto-Oncogene Proteins, Receptors, G-Protein-Coupled, Sequence Homology, Amino Acid, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
Abstract | <p>During cell division, polarized epithelial cells employ mechanisms to preserve cell polarity and tissue integrity. In dividing cells of the mammalian skin, planar cell polarity (PCP) is maintained through the bulk internalization, equal segregation, and polarized recycling of cortical PCP proteins. The dramatic redistribution of PCP proteins coincides precisely with cell-cycle progression, but the mechanisms coordinating PCP and mitosis are unknown. Here we identify Plk1 as a master regulator of PCP dynamics during mitosis. Plk1 interacts with core PCP component Celsr1 via a conserved polo-box domain (PBD)-binding motif, localizes to mitotic endosomes, and directly phosphorylates Celsr1. Plk1-dependent phosphorylation activates the endocytic motif specifically during mitosis, allowing bulk recruitment of Celsr1 into endosomes. Inhibiting Plk1 activity blocks PCP internalization and perturbs PCP asymmetry. Mimicking dileucine motif phosphorylation is sufficient to drive Celsr1 internalization during interphase. Thus, Plk1-mediated phosphorylation of Celsr1 ensures that PCP redistribution is precisely coordinated with mitotic entry.</p> |
DOI | 10.1016/j.devcel.2015.03.024 |
Alternate Journal | Dev Cell |
PubMed ID | 26004507 |
PubMed Central ID | PMC4464975 |
Grant List | R00 AR057501 / AR / NIAMS NIH HHS / United States R00AR057501 / AR / NIAMS NIH HHS / United States |