Minibrain and Wings apart control organ growth and tissue patterning through down-regulation of Capicua.

TitleMinibrain and Wings apart control organ growth and tissue patterning through down-regulation of Capicua.
Publication TypeJournal Article
Year of Publication2016
AuthorsYang, L, Paul, S, Trieu, KG, Dent, LG, Froldi, F, Forés, M, Webster, K, Siegfried, KR, Kondo, S, Harvey, K, Cheng, L, Jiménez, G, Shvartsman, SY, Veraksa, A
JournalProc Natl Acad Sci U S A
Volume113
Issue38
Pagination10583-8
Date Published2016 09 20
ISSN1091-6490
Abstract

The transcriptional repressor Capicua (Cic) controls tissue patterning and restricts organ growth, and has been recently implicated in several cancers. Cic has emerged as a primary sensor of signaling downstream of the receptor tyrosine kinase (RTK)/extracellular signal-regulated kinase (ERK) pathway, but how Cic activity is regulated in different cellular contexts remains poorly understood. We found that the kinase Minibrain (Mnb, ortholog of mammalian DYRK1A), acting through the adaptor protein Wings apart (Wap), physically interacts with and phosphorylates the Cic protein. Mnb and Wap inhibit Cic function by limiting its transcriptional repressor activity. Down-regulation of Cic by Mnb/Wap is necessary for promoting the growth of multiple organs, including the wings, eyes, and the brain, and for proper tissue patterning in the wing. We have thus uncovered a previously unknown mechanism of down-regulation of Cic activity by Mnb and Wap, which operates independently from the ERK-mediated control of Cic. Therefore, Cic functions as an integrator of upstream signals that are essential for tissue patterning and organ growth. Finally, because DYRK1A and CIC exhibit, respectively, prooncogenic vs. tumor suppressor activities in human oligodendroglioma, our results raise the possibility that DYRK1A may also down-regulate CIC in human cells.

DOI10.1073/pnas.1609417113
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID27601662
PubMed Central IDPMC5035877