Metabolomics and Isotope Tracing.

TitleMetabolomics and Isotope Tracing.
Publication TypeJournal Article
Year of Publication2018
AuthorsJang, C, Chen, L, Rabinowitz, JD
JournalCell
Volume173
Issue4
Pagination822-837
Date Published2018 05 03
ISSN1097-4172
KeywordsChromatography, High Pressure Liquid, Glycolysis, Humans, Isotope Labeling, Mass Spectrometry, Metabolome, Metabolomics, Mitochondria, Principal Component Analysis
Abstract

<p>Great strides have been made over the past decade toward comprehensive study of metabolism. Mass spectrometry (MS) has played a central role by enabling measurement of many metabolites simultaneously. Tracking metabolite labeling from stable isotope tracers can in addition reveal pathway activities. Here, we describe the basics of metabolite measurement by MS, including sample preparation, metabolomic analysis, and data interpretation. In addition, drawing on examples of successful experiments, we highlight the ways in which metabolomics and isotope tracing can illuminate biology.</p>

DOI10.1016/j.cell.2018.03.055
Alternate JournalCell
PubMed ID29727671
PubMed Central IDPMC6034115
Grant ListDP1 DK113643 / DK / NIDDK NIH HHS / United States
P30 DK019525 / DK / NIDDK NIH HHS / United States
R01 CA163591 / CA / NCI NIH HHS / United States
P30 CA072720 / CA / NCI NIH HHS / United States