Metabolomics and Isotope Tracing.

TitleMetabolomics and Isotope Tracing.
Publication TypeJournal Article
Year of Publication2018
AuthorsJang, C, Chen, L, Rabinowitz, JD
JournalCell
Volume173
Issue4
Pagination822-837
Date Published2018 May 03
ISSN1097-4172
Abstract

Great strides have been made over the past decade toward comprehensive study of metabolism. Mass spectrometry (MS) has played a central role by enabling measurement of many metabolites simultaneously. Tracking metabolite labeling from stable isotope tracers can in addition reveal pathway activities. Here, we describe the basics of metabolite measurement by MS, including sample preparation, metabolomic analysis, and data interpretation. In addition, drawing on examples of successful experiments, we highlight the ways in which metabolomics and isotope tracing can illuminate biology.

DOI10.1016/j.cell.2018.03.055
Alternate JournalCell
PubMed ID29727671
Grant ListDP1 DK113643 / DK / NIDDK NIH HHS / United States
R01 CA163591 / CA / NCI NIH HHS / United States