|Title||Metabolomics and Isotope Tracing.|
|Publication Type||Journal Article|
|Year of Publication||2018|
|Authors||Jang, C, Chen, L, Rabinowitz, JD|
|Date Published||2018 May 03|
Great strides have been made over the past decade toward comprehensive study of metabolism. Mass spectrometry (MS) has played a central role by enabling measurement of many metabolites simultaneously. Tracking metabolite labeling from stable isotope tracers can in addition reveal pathway activities. Here, we describe the basics of metabolite measurement by MS, including sample preparation, metabolomic analysis, and data interpretation. In addition, drawing on examples of successful experiments, we highlight the ways in which metabolomics and isotope tracing can illuminate biology.
|Grant List||DP1 DK113643 / DK / NIDDK NIH HHS / United States |
R01 CA163591 / CA / NCI NIH HHS / United States