Metabolite Exchange between Mammalian Organs Quantified in Pigs. Author Cholsoon Jang, Sheng Hui, Xianfeng Zeng, Alexis Cowan, Lin Wang, Li Chen, Raphael Morscher, Jorge Reyes, Christian Frezza, Ho Hwang, Akito Imai, Yoshiaki Saito, Keitaro Okamoto, Christine Vaspoli, Loewe Kasprenski, Gerald Zsido, Joseph Gorman, Robert Gorman, Joshua Rabinowitz Publication Year 2019 Type Journal Article Abstract Mammalian organs continually exchange metabolites via circulation, but systems-level analysis of this shuttling process is lacking. Here, we compared, in fasted pigs, metabolite concentrations in arterial blood versus draining venous blood from 11 organs. Greater than 90% of metabolites showed arterial-venous differences across at least one organ. Surprisingly, the liver and kidneys released not only glucose but also amino acids, both of which were consumed primarily by the intestine and pancreas. The liver and kidneys exhibited additional unexpected activities: liver preferentially burned unsaturated over more atherogenic saturated fatty acids, whereas the kidneys were unique in burning circulating citrate and net oxidizing lactate to pyruvate, thereby contributing to circulating redox homeostasis. Furthermore, we observed more than 700 other cases of tissue-specific metabolite production or consumption, such as release of nucleotides by the spleen and TCA intermediates by pancreas. These data constitute a high-value resource, providing a quantitative atlas of inter-organ metabolite exchange. Keywords Animals, Mice, Fatty Acids, Mice, Inbred C57BL, Lactic Acid, Male, Kidney, Amino Acids, Liver, Swine, Pyruvic Acid, Intestinal Mucosa, Blood Glucose, Fasting, Spleen, Citric Acid, Arteries, Pancreas, Veins Journal Cell Metab Volume 30 Issue 3 Pages 594-606.e3 Date Published 2019 Sep 03 ISSN Number 1932-7420 DOI 10.1016/j.cmet.2019.06.002 Alternate Journal Cell Metab PMCID PMC6726553 PMID 31257152 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML