A Metabolic Pathway for Activation of Dietary Glucosinolates by a Human Gut Symbiont.

TitleA Metabolic Pathway for Activation of Dietary Glucosinolates by a Human Gut Symbiont.
Publication TypeJournal Article
Year of Publication2020
AuthorsLiou, CS, Sirk, SJ, Diaz, CAC, Klein, AP, Fischer, CR, Higginbottom, SK, Erez, A, Donia, MS, Sonnenburg, JL, Sattely, ES
Date Published2020 02 20
KeywordsAnimals, Bacteroides thetaiotaomicron, Dietary Carbohydrates, Gene Expression Regulation, Bacterial, Glucosinolates, Humans, Intestines, Male, Mice, Operon, Symbiosis

<p>Consumption of glucosinolates, pro-drug-like metabolites abundant in Brassica vegetables, has been associated with decreased risk of certain cancers. Gut microbiota have the ability to metabolize glucosinolates, generating chemopreventive isothiocyanates. Here, we identify a genetic and biochemical basis for activation of glucosinolates to isothiocyanates by Bacteroides thetaiotaomicron, a prominent gut commensal species. Using a genome-wide transposon insertion screen, we identified an operon required for glucosinolate metabolism in B. thetaiotaomicron. Expression of BT2159-BT2156 in a non-metabolizing relative, Bacteroides fragilis, resulted in gain of glucosinolate metabolism. We show that isothiocyanate formation requires the action of BT2158 and either BT2156 or BT2157 in vitro. Monocolonization of mice with mutant BtΔ2157 showed reduced isothiocyanate production in the gastrointestinal tract. These data provide insight into the mechanisms by which a common gut bacterium processes an important dietary nutrient.</p>

Alternate JournalCell
PubMed ID32084341
PubMed Central IDPMC7515767
Grant ListDP2 AT008321 / AT / NCCIH NIH HHS / United States
R01 DK085025 / DK / NIDDK NIH HHS / United States
T32 GM120007 / GM / NIGMS NIH HHS / United States
/ HHMI / Howard Hughes Medical Institute / United States