Mechanisms of Mitotic Spindle Assembly.

TitleMechanisms of Mitotic Spindle Assembly.
Publication TypeJournal Article
Year of Publication2016
AuthorsPetry, S
JournalAnnu Rev Biochem
Date Published2016 Jun 02
KeywordsAnimals, Centrosome, Chromosome Segregation, Drosophila melanogaster, Gene Expression Regulation, Humans, Kinesins, Kinetochores, Metaphase, Microtubule-Associated Proteins, Microtubules, Signal Transduction, Spindle Apparatus, Tubulin, Xenopus laevis, Xenopus Proteins, Zygote

<p>Life depends on cell proliferation and the accurate segregation of chromosomes, which are mediated by the microtubule (MT)-based mitotic spindle and ∼200 essential MT-associated proteins. Yet, a mechanistic understanding of how the mitotic spindle is assembled and achieves chromosome segregation is still missing. This is mostly due to the density of MTs in the spindle, which presumably precludes their direct observation. Recent insight has been gained into the molecular building plan of the metaphase spindle using bulk and single-molecule measurements combined with computational modeling. MT nucleation was uncovered as a key principle of spindle assembly, and mechanistic details about MT nucleation pathways and their coordination are starting to be revealed. Lastly, advances in studying spindle assembly can be applied to address the molecular mechanisms of how the spindle segregates chromosomes.</p>

Alternate JournalAnnu Rev Biochem
PubMed ID27145846
PubMed Central IDPMC5016079
Grant ListDP2 GM123493 / GM / NIGMS NIH HHS / United States
R00 GM100013 / GM / NIGMS NIH HHS / United States