The mechanism of Na⁺/K⁺ selectivity in mammalian voltage-gated sodium channels based on molecular dynamics simulation.

TitleThe mechanism of Na⁺/K⁺ selectivity in mammalian voltage-gated sodium channels based on molecular dynamics simulation.
Publication TypeJournal Article
Year of Publication2013
AuthorsXia, M, Liu, H, Li, Y, Yan, N, Gong, H
JournalBiophys J
Volume104
Issue11
Pagination2401-9
Date Published2013 Jun 04
ISSN1542-0086
KeywordsAmino Acid Sequence, Molecular Dynamics Simulation, Mutation, Permeability, Potassium, Protein Conformation, Sodium, Substrate Specificity, Voltage-Gated Sodium Channels
Abstract

Voltage-gated sodium (Nav) channels and their Na⁺/K⁺ selectivity are of great importance in the mammalian neuronal signaling. According to mutational analysis, the Na⁺/K⁺ selectivity in mammalian Nav channels is mainly determined by the Lys and Asp/Glu residues located at the constriction site within the selectivity filter. Despite successful molecular dynamics simulations conducted on the prokaryotic Nav channels, the lack of Lys at the constriction site of prokaryotic Nav channels limits how much can be learned about the Na⁺/K⁺ selectivity in mammalian Nav channels. In this work, we modeled the mammalian Nav channel by mutating the key residues at the constriction site in a prokaryotic Nav channel (NavRh) to its mammalian counterpart. By simulating the mutant structure, we found that the Na⁺ preference in mammalian Nav channels is collaboratively achieved by the deselection from Lys and the selection from Asp/Glu within the constriction site.

DOI10.1016/j.bpj.2013.04.035
Alternate JournalBiophys. J.
PubMed ID23746512
PubMed Central IDPMC3672897