Mechanism of bidirectional thermotaxis in .

TitleMechanism of bidirectional thermotaxis in .
Publication TypeJournal Article
Year of Publication2017
AuthorsPaulick, A, Jakovljevic, V, Zhang, SM, Erickstad, M, Groisman, A, Meir, Y, Ryu, WS, Wingreen, NS, Sourjik, V
Date Published2017 Aug 03
KeywordsAdaptation, Physiological, Aspartic Acid, Bacterial Proteins, Chemotactic Factors, Escherichia coli K12, Escherichia coli Proteins, Fluorescence Resonance Energy Transfer, Gene Expression Regulation, Bacterial, Green Fluorescent Proteins, Luminescent Proteins, Methyl-Accepting Chemotaxis Proteins, Microfluidic Analytical Techniques, Receptors, Cell Surface, Recombinant Fusion Proteins, Serine, Signal Transduction, Taxis Response, Temperature

<p>In bacteria various tactic responses are mediated by the same cellular pathway, but sensing of physical stimuli remains poorly understood. Here, we combine an in-vivo analysis of the pathway activity with a microfluidic taxis assay and mathematical modeling to investigate the thermotactic response of . We show that in the absence of chemical attractants exhibits a steady thermophilic response, the magnitude of which decreases at higher temperatures. Adaptation of wild-type cells to high levels of chemoattractants sensed by only one of the major chemoreceptors leads to inversion of the thermotactic response at intermediate temperatures and bidirectional cell accumulation in a thermal gradient. A mathematical model can explain this behavior based on the saturation-dependent kinetics of adaptive receptor methylation. Lastly, we find that the preferred accumulation temperature corresponds to optimal growth in the presence of the chemoattractant serine, pointing to a physiological relevance of the observed thermotactic behavior.</p>

Alternate JournalElife
PubMed ID28826491
PubMed Central IDPMC5578741
Grant ListR01 GM082938 / GM / NIGMS NIH HHS / United States