Mechanics of a multilayer epithelium instruct tumour architecture and function.

TitleMechanics of a multilayer epithelium instruct tumour architecture and function.
Publication TypeJournal Article
Year of Publication2020
AuthorsFiore, VF, Krajnc, M, Quiroz, FGarcia, Levorse, J, H Pasolli, A, Shvartsman, SY, Fuchs, E
JournalNature
Volume585
Issue7825
Pagination433-439
Date Published2020 09
ISSN1476-4687
KeywordsActomyosin, Animals, Basement Membrane, Carcinogenesis, Carcinoma, Basal Cell, Carcinoma, Squamous Cell, Cell Proliferation, Computer Simulation, Disease Progression, Epithelial Cells, Extracellular Matrix, Female, Humans, Mice, Neoplasm Invasiveness, Pliability
Abstract

<p>Loss of normal tissue architecture is a hallmark of oncogenic transformation. In developing organisms, tissues architectures are sculpted by mechanical forces during morphogenesis. However, the origins and consequences of tissue architecture during tumorigenesis remain elusive. In skin, premalignant basal cell carcinomas form 'buds', while invasive squamous cell carcinomas initiate as 'folds'. Here, using computational modelling, genetic manipulations and biophysical measurements, we identify the biophysical underpinnings and biological consequences of these tumour architectures. Cell proliferation and actomyosin contractility dominate tissue architectures in monolayer, but not multilayer, epithelia. In stratified epidermis, meanwhile, softening and enhanced remodelling of the basement membrane promote tumour budding, while stiffening of the basement membrane promotes folding. Additional key forces stem from the stratification and differentiation of progenitor cells. Tumour-specific suprabasal stiffness gradients are generated as oncogenic lesions progress towards malignancy, which we computationally predict will alter extensile tensions on the tumour basement membrane. The pathophysiologic ramifications of this prediction are profound. Genetically decreasing the stiffness of basement membranes increases membrane tensions in silico and potentiates the progression of invasive squamous cell carcinomas in vivo. Our findings suggest that mechanical forces-exerted from above and below progenitors of multilayered epithelia-function to shape premalignant tumour architectures and influence tumour progression.</p>

DOI10.1038/s41586-020-2695-9
Alternate JournalNature
PubMed ID32879493
PubMed Central IDPMC7787055
Grant ListR37 AR027883 / AR / NIAMS NIH HHS / United States
/ WT_ / Wellcome Trust / United Kingdom
/ HHMI / Howard Hughes Medical Institute / United States
CA009673-39 / NH / NIH HHS / United States
T32 CA009673 / CA / NCI NIH HHS / United States
R01 AR027883 / AR / NIAMS NIH HHS / United States