Mechanical Frustration of Phase Separation in the Cell Nucleus by Chromatin.

TitleMechanical Frustration of Phase Separation in the Cell Nucleus by Chromatin.
Publication TypeJournal Article
Year of Publication2021
AuthorsZhang, Y, Lee, DSW, Meir, Y, Brangwynne, CP, Wingreen, NS
JournalPhys Rev Lett
Volume126
Issue25
Pagination258102
Date Published2021 Jun 25
ISSN1079-7114
KeywordsBiomechanical Phenomena, Cell Line, Tumor, Cell Nucleus, Chromatin, Humans, Models, Chemical, Phase Transition, Surface Properties, Thermodynamics
Abstract

<p>Liquid-liquid phase separation is a fundamental mechanism underlying subcellular organization. Motivated by the striking observation that optogenetically generated droplets in the nucleus display suppressed coarsening dynamics, we study the impact of chromatin mechanics on droplet phase separation. We combine theory and simulation to show that cross-linked chromatin can mechanically suppress droplets' coalescence and ripening, as well as quantitatively control their number, size, and placement. Our results highlight the role of the subcellular mechanical environment on condensate regulation.</p>

DOI10.1103/PhysRevLett.126.258102
Alternate JournalPhys Rev Lett
PubMed ID34241518