Measurement of cortical elasticity in Drosophila melanogaster embryos using ferrofluids. Author Konstantin Doubrovinski, Michael Swan, Oleg Polyakov, Eric Wieschaus Publication Year 2017 Type Journal Article Abstract Many models of morphogenesis are forced to assume specific mechanical properties of cells, because the actual mechanical properties of living tissues are largely unknown. Here, we measure the rheology of epithelial cells in the cellularizing Drosophila embryo by injecting magnetic particles and studying their response to external actuation. We establish that, on timescales relevant to epithelial morphogenesis, the cytoplasm is predominantly viscous, whereas the cellular cortex is elastic. The timescale of elastic stress relaxation has a lower bound of 4 min, which is comparable to the time required for internalization of the ventral furrow during gastrulation. The cytoplasm was measured to be ∼10-fold as viscous as water. We show that elasticity depends on the actin cytoskeleton and conclude by discussing how these results relate to existing mechanical models of morphogenesis. Keywords Animals, Morphogenesis, Models, Biological, Drosophila melanogaster, Stress, Mechanical, Embryo, Nonmammalian, Epithelial Cells, Cytoskeleton, Cytoplasm, Rheology, Gastrulation, Elasticity, Viscosity, Magnetite Nanoparticles, Giant Cells, Magnetics, Magnets, Microinjections Journal Proc Natl Acad Sci U S A Volume 114 Issue 5 Pages 1051-1056 Date Published 2017 Jan 31 ISSN Number 1091-6490 DOI 10.1073/pnas.1616659114 Alternate Journal Proc Natl Acad Sci U S A PMCID PMC5293093 PMID 28096360 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML