Title | Mapping of functional elements of the Fab-6 boundary involved in the regulation of the Abd-B hox gene in Drosophila melanogaster. |
Publication Type | Journal Article |
Year of Publication | 2021 |
Authors | Postika, N, Schedl, P, Georgiev, P, Kyrchanova, O |
Journal | Sci Rep |
Volume | 11 |
Issue | 1 |
Pagination | 4156 |
Date Published | 2021 Feb 18 |
ISSN | 2045-2322 |
Keywords | Animals, Drosophila melanogaster, Drosophila Proteins, Gene Deletion, Genes, Homeobox, Genes, Insect, Insulator Elements, Polycomb-Group Proteins, Response Elements |
Abstract | <p>The autonomy of segment-specific regulatory domains in the Bithorax complex is conferred by boundary elements and associated Polycomb response elements (PREs). The Fab-6 boundary is located at the junction of the iab-5 and iab-6 domains. Previous studies mapped it to a nuclease hypersensitive region 1 (HS1), while the iab-6 PRE was mapped to a second hypersensitive region HS2 nearly 3 kb away. To analyze the role of HS1 and HS2 in boundary we generated deletions of HS1 or HS1 + HS2 that have attP site for boundary replacement experiments. The 1389 bp HS1 deletion can be rescued by a 529 bp core Fab-6 sequence that includes two CTCF sites. However, Fab-6 HS1 cannot rescue the HS1 + HS2 deletion or substitute for another BX-C boundary - Fab-7. For this it must be combined with a PRE, either Fab-7 HS3, or Fab-6 HS2. These findings suggest that the boundary function of Fab-6 HS1 must be bolstered by a second element that has PRE activity.</p> |
DOI | 10.1038/s41598-021-83734-8 |
Alternate Journal | Sci Rep |
PubMed ID | 33603202 |
PubMed Central ID | PMC7892861 |
Grant List | R35 GM126975 / GM / NIGMS NIH HHS / United States R35 GM126975 / GF / NIH HHS / United States |