Mapping of functional elements of the Fab-6 boundary involved in the regulation of the Abd-B hox gene in Drosophila melanogaster.

TitleMapping of functional elements of the Fab-6 boundary involved in the regulation of the Abd-B hox gene in Drosophila melanogaster.
Publication TypeJournal Article
Year of Publication2021
AuthorsPostika, N, Schedl, P, Georgiev, P, Kyrchanova, O
JournalSci Rep
Volume11
Issue1
Pagination4156
Date Published2021 Feb 18
ISSN2045-2322
Abstract

<p>The autonomy of segment-specific regulatory domains in the Bithorax complex is conferred by boundary elements and associated Polycomb response elements (PREs). The Fab-6 boundary is located at the junction of the iab-5 and iab-6 domains. Previous studies mapped it to a nuclease hypersensitive region 1 (HS1), while the iab-6 PRE was mapped to a second hypersensitive region HS2 nearly 3 kb away. To analyze the role of HS1 and HS2 in boundary we generated deletions of HS1 or HS1 + HS2 that have attP site for boundary replacement experiments. The 1389 bp HS1 deletion can be rescued by a 529 bp core Fab-6 sequence that includes two CTCF sites. However, Fab-6 HS1 cannot rescue the HS1 + HS2 deletion or substitute for another BX-C boundary - Fab-7. For this it must be combined with a PRE, either Fab-7 HS3, or Fab-6 HS2. These findings suggest that the boundary function of Fab-6 HS1 must be bolstered by a second element that has PRE activity.</p>

DOI10.1038/s41598-021-83734-8
Alternate JournalSci Rep
PubMed ID33603202
PubMed Central IDPMC7892861
Grant List19-14-00103 / / Russian Science Foundation /
R35 GM126975 / GF / NIH HHS / United States
075-15-2019-1661 / / Ministry of Science and Higher Education of the Russian Federation /