LuxT controls specific quorum-sensing-regulated behaviors in Vibrionaceae spp. via repression of qrr1, encoding a small regulatory RNA.

TitleLuxT controls specific quorum-sensing-regulated behaviors in Vibrionaceae spp. via repression of qrr1, encoding a small regulatory RNA.
Publication TypeJournal Article
Year of Publication2021
AuthorsEickhoff, MJ, Fei, C, Huang, X, Bassler, BL
JournalPLoS Genet
Volume17
Issue4
Paginatione1009336
Date Published2021 04
ISSN1553-7404
KeywordsBacterial Proteins, DNA-Binding Proteins, Escherichia coli, Genes, Regulator, Phylogeny, Quorum Sensing, Regulatory Sequences, Ribonucleic Acid, RNA, Messenger, Signal Transduction, Vibrio cholerae, Vibrionaceae
Abstract

<p>Quorum sensing (QS) is a process of chemical communication bacteria use to transition between individual and collective behaviors. QS depends on the production, release, and synchronous response to signaling molecules called autoinducers (AIs). The marine bacterium Vibrio harveyi monitors AIs using a signal transduction pathway that relies on five small regulatory RNAs (called Qrr1-5) that post-transcriptionally control target genes. Curiously, the small RNAs largely function redundantly making it difficult to understand the necessity for five of them. Here, we identify LuxT as a transcriptional repressor of qrr1. LuxT does not regulate qrr2-5, demonstrating that qrr genes can be independently controlled to drive unique downstream QS gene expression patterns. LuxT reinforces its control over the same genes it regulates indirectly via repression of qrr1, through a second transcriptional control mechanism. Genes dually regulated by LuxT specify public goods including an aerolysin-type pore-forming toxin. Phylogenetic analyses reveal that LuxT is conserved among Vibrionaceae and sequence comparisons predict that LuxT represses qrr1 in additional species. The present findings reveal that the QS regulatory RNAs can carry out both shared and unique functions to endow bacteria with plasticity in their output behaviors.</p>

DOI10.1371/journal.pgen.1009336
Alternate JournalPLoS Genet
PubMed ID33793568
PubMed Central IDPMC8043402
Grant ListR37 GM065859 / GM / NIGMS NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States
/ HHMI / Howard Hughes Medical Institute / United States