Localized production of defence chemicals by intracellular symbionts of Haliclona sponges.

TitleLocalized production of defence chemicals by intracellular symbionts of Haliclona sponges.
Publication TypeJournal Article
Year of Publication2019
AuthorsTianero, MDiarey, Balaich, JN, Donia, MS
JournalNat Microbiol
Date Published2019 Jul
KeywordsAnimals, Gammaproteobacteria, Genome Size, Haliclona, Host Specificity, Metagenome, Molecular Structure, Multigene Family, Phylogeny, Plasmids, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Symbiosis, Tetrahydroisoquinolines

<p>Marine sponges often house small-molecule-producing symbionts extracellularly in their mesohyl, providing the host with a means of chemical defence against predation and microbial infection. Here, we report an intriguing case of chemically mediated symbiosis between the renieramycin-containing sponge Haliclona sp. and its herein discovered renieramycin-producing symbiont Candidatus Endohaliclona renieramycinifaciens. Remarkably, Ca. E. renieramycinifaciens has undergone extreme genome reduction where it has lost almost all necessary elements for free living while maintaining a complex, multi-copy plasmid-encoded biosynthetic gene cluster for renieramycin biosynthesis. In return, the sponge houses Ca. E. renieramycinifaciens in previously uncharacterized cellular reservoirs (chemobacteriocytes), where it can acquire nutrients from the host and avoid bacterial competition. This relationship is highly specific to a single clade of Haliclona sponges. Our study reveals intracellular symbionts as an understudied source for defence chemicals in the oldest-living metazoans and paves the way towards discovering similar systems in other marine sponges.</p>

Alternate JournalNat Microbiol
PubMed ID30936484
PubMed Central IDPMC8647704
Grant ListDP2 AI124441 / AI / NIAID NIH HHS / United States
P30 CA072720 / CA / NCI NIH HHS / United States