Live Imaging of mRNA Synthesis in Drosophila.

TitleLive Imaging of mRNA Synthesis in Drosophila.
Publication TypeJournal Article
Year of Publication2018
AuthorsGarcia, HG, Gregor, T
JournalMethods Mol Biol
Volume1649
Pagination349-357
Date Published2018
ISSN1940-6029
KeywordsAnimals, Animals, Genetically Modified, Calibration, DNA, Drosophila melanogaster, Embryo, Nonmammalian, Genes, Reporter, Imaging, Three-Dimensional, Microscopy, Fluorescence, Multiphoton, RNA Polymerase II, RNA, Messenger
Abstract

<p>mRNA synthesis is one of the earliest readouts of the activity of a transcribed gene, which is of particular interest in the context of metazoan cell fate specification. These processes are intrinsically dynamic and stochastic, which makes in vivo single-cell measurements inevitable. Here, we present the application of a technology that has been widely used in single celled organisms to measure transcriptional activity in developing embryos of the fruit fly Drosophila melanogaster. The method allows for quantification of instantaneous polymerase occupancy of active gene loci and thereby enables the development and testing of models of gene regulation in development.</p>

DOI10.1007/978-1-4939-7213-5_23
Alternate JournalMethods Mol. Biol.
PubMed ID29130209
PubMed Central IDPMC5909709
Grant ListP50 GM071508 / GM / NIGMS NIH HHS / United States
R01 GM097275 / GM / NIGMS NIH HHS / United States
U01 EB021239 / EB / NIBIB NIH HHS / United States