Lipopolysaccharide transport and assembly at the outer membrane: the PEZ model. Author Suguru Okuda, David Sherman, Thomas Silhavy, Natividad Ruiz, Daniel Kahne Publication Year 2016 Type Journal Article Abstract Gram-negative bacteria have a double-membrane cellular envelope that enables them to colonize harsh environments and prevents the entry of many clinically available antibiotics. A main component of most outer membranes is lipopolysaccharide (LPS), a glycolipid containing several fatty acyl chains and up to hundreds of sugars that is synthesized in the cytoplasm. In the past two decades, the proteins that are responsible for transporting LPS across the cellular envelope and assembling it at the cell surface in Escherichia coli have been identified, but it remains unclear how they function. In this Review, we discuss recent advances in this area and present a model that explains how energy from the cytoplasm is used to power LPS transport across the cellular envelope to the cell surface. Keywords Escherichia coli, Biological Transport, Models, Biological, Escherichia coli Proteins, Cell Membrane, Bacterial Outer Membrane Proteins, Metabolic Networks and Pathways, Lipopolysaccharides Journal Nat Rev Microbiol Volume 14 Issue 6 Pages 337-45 Date Published 2016 Jun ISSN Number 1740-1534 DOI 10.1038/nrmicro.2016.25 Alternate Journal Nat Rev Microbiol PMCID PMC4937791 PMID 27026255 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML