Lipopolysaccharide transport and assembly at the outer membrane: the PEZ model.

TitleLipopolysaccharide transport and assembly at the outer membrane: the PEZ model.
Publication TypeJournal Article
Year of Publication2016
AuthorsOkuda, S, Sherman, DJ, Silhavy, TJ, Ruiz, N, Kahne, D
JournalNat Rev Microbiol
Volume14
Issue6
Pagination337-45
Date Published2016 06 01
ISSN1740-1534
Abstract

Gram-negative bacteria have a double-membrane cellular envelope that enables them to colonize harsh environments and prevents the entry of many clinically available antibiotics. A main component of most outer membranes is lipopolysaccharide (LPS), a glycolipid containing several fatty acyl chains and up to hundreds of sugars that is synthesized in the cytoplasm. In the past two decades, the proteins that are responsible for transporting LPS across the cellular envelope and assembling it at the cell surface in Escherichia coli have been identified, but it remains unclear how they function. In this Review, we discuss recent advances in this area and present a model that explains how energy from the cytoplasm is used to power LPS transport across the cellular envelope to the cell surface.

DOI10.1038/nrmicro.2016.25
Alternate JournalNat. Rev. Microbiol.
PubMed ID27026255
PubMed Central IDPMC4937791
Grant ListR01 AI081059 / AI / NIAID NIH HHS / United States
R01 GM034821 / GM / NIGMS NIH HHS / United States
R01 GM100951 / GM / NIGMS NIH HHS / United States
R37 GM034821 / GM / NIGMS NIH HHS / United States