Title | Light-based feedback for controlling intracellular signaling dynamics. |
Publication Type | Journal Article |
Year of Publication | 2011 |
Authors | Toettcher, JE, Gong, D, Lim, WA, Weiner, OD |
Journal | Nat Methods |
Volume | 8 |
Issue | 10 |
Pagination | 837-9 |
Date Published | 2011 Sep 11 |
ISSN | 1548-7105 |
Keywords | Animals, Cell Membrane, Computational Biology, Feedback, Physiological, Humans, Light, Models, Biological, Phosphatidylinositol 3-Kinases, Phosphoinositide-3 Kinase Inhibitors, Protein Binding, Signal Transduction |
Abstract | <p>The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.</p> |
DOI | 10.1038/nmeth.1700 |
Alternate Journal | Nat Methods |
PubMed ID | 21909100 |
PubMed Central ID | PMC3184382 |
Grant List | R01 GM062583-08 / GM / NIGMS NIH HHS / United States P50 GM081879-01A2 / GM / NIGMS NIH HHS / United States PN2 EY016546-06 / EY / NEI NIH HHS / United States GM55040 / GM / NIGMS NIH HHS / United States R01 GM084040 / GM / NIGMS NIH HHS / United States P50GM081879 / GM / NIGMS NIH HHS / United States P50 GM081879 / GM / NIGMS NIH HHS / United States R01 GM084040-04 / GM / NIGMS NIH HHS / United States PN1 EY016546 / EY / NEI NIH HHS / United States R01 GM062583 / GM / NIGMS NIH HHS / United States R01 GM055040 / GM / NIGMS NIH HHS / United States R01 GM096164 / GM / NIGMS NIH HHS / United States GM62583 / GM / NIGMS NIH HHS / United States GM084040 / GM / NIGMS NIH HHS / United States PN2 EY016546 / EY / NEI NIH HHS / United States / / Howard Hughes Medical Institute / United States R01 GM055040-12 / GM / NIGMS NIH HHS / United States EY016546 / EY / NEI NIH HHS / United States |