Title | JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Albig, C, Wang, C, Dann, GP, Wojcik, F, Schauer, T, Krause, S, Maenner, S, Cai, W, Li, Y, Girton, J, Muir, TW, Johansen, J, Johansen, KM, Becker, PB, Regnard, C |
Journal | Nat Commun |
Volume | 10 |
Issue | 1 |
Pagination | 5343 |
Date Published | 2019 Nov 25 |
ISSN | 2041-1723 |
Keywords | Animals, Cell Line, Chromatin, Chromatin Assembly and Disassembly, Drosophila melanogaster, Drosophila Proteins, Heterochromatin, Histones, Humans, Interphase, Methylation, Phosphorylation, Protein Processing, Post-Translational, Protein Serine-Threonine Kinases |
Abstract | <p>In flies, the chromosomal kinase JIL-1 is responsible for most interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from acquiring heterochromatic marks, such as dimethylated histone H3K9 (H3K9me2) and HP1. Here, we show that JIL-1's targeting to chromatin depends on a PWWP domain-containing protein JASPer (JIL-1 Anchoring and Stabilizing Protein). JASPer-JIL-1 (JJ)-complex is the major form of kinase in vivo and is targeted to active genes and telomeric transposons via binding of the PWWP domain of JASPer to H3K36me3 nucleosomes, to modulate transcriptional output. JIL-1 and JJ-complex depletion in cycling cells lead to small changes in H3K9me2 distribution at active genes and telomeric transposons. Finally, we identify interactors of the endogenous JJ-complex and propose that JIL-1 not only prevents heterochromatin formation but also coordinates chromatin-based regulation in the transcribed part of the genome.</p> |
DOI | 10.1038/s41467-019-13174-6 |
Alternate Journal | Nat Commun |
PubMed ID | 31767855 |
PubMed Central ID | PMC6877644 |
Grant List | R01 GM062916 / GM / NIGMS NIH HHS / United States R01 GM086868 / GM / NIGMS NIH HHS / United States R01 GM107047 / GM / NIGMS NIH HHS / United States R37 GM086868 / GM / NIGMS NIH HHS / United States |