Inverse regulation of biofilm dispersal by polyamine signals.

TitleInverse regulation of biofilm dispersal by polyamine signals.
Publication TypeJournal Article
Year of Publication2021
AuthorsBridges, AA, Bassler, BL
JournalElife
Volume10
Date Published2021 04 15
ISSN2050-084X
KeywordsBacterial Proteins, Biofilms, Polyamines, Signal Transduction, Vibrio cholerae
Abstract

<p>The global pathogen undergoes cycles of biofilm formation and dispersal in the environment and the human host. Little is understood about biofilm dispersal. Here, we show that MbaA, a periplasmic polyamine sensor, and PotD1, a polyamine importer, regulate biofilm dispersal. Spermidine, a commonly produced polyamine, drives dispersal, whereas norspermidine, an uncommon polyamine produced by vibrios, inhibits dispersal. Spermidine and norspermidine differ by one methylene group. Both polyamines control dispersal via MbaA detection in the periplasm and subsequent signal relay. Our results suggest that dispersal fails in the absence of PotD1 because endogenously produced norspermidine is not reimported, periplasmic norspermidine accumulates, and it stimulates MbaA signaling. These results suggest that uses MbaA to monitor environmental polyamines, blends of which potentially provide information about numbers of 'self' and 'other'. This information is used to dictate whether or not to disperse from biofilms.</p>

DOI10.7554/eLife.65487
Alternate JournalElife
PubMed ID33856344
PubMed Central IDPMC8079147
Grant ListDRG-2302-17 / DRCRF / Damon Runyon Cancer Research Foundation / United States
5R37GM065859 / NH / NIH HHS / United States
/ HHMI / Howard Hughes Medical Institute / United States
MCB-1713731 / / National Science Foundation /
R37 GM065859 / GM / NIGMS NIH HHS / United States