Title | Interrogating the transcriptome with metabolically incorporated ribonucleosides. |
Publication Type | Journal Article |
Year of Publication | 2021 |
Authors | Kleiner, RE |
Journal | Mol Omics |
Volume | 17 |
Issue | 6 |
Pagination | 833-841 |
Date Published | 2021 12 06 |
ISSN | 2515-4184 |
Keywords | High-Throughput Nucleotide Sequencing, Mass Spectrometry, Ribonucleosides, RNA, Transcriptome |
Abstract | <p>RNA is a central player in biological processes, but there remain major gaps in our understanding of transcriptomic processes and the underlying biochemical mechanisms regulating RNA in cells. A powerful strategy to facilitate molecular analysis of cellular RNA is the metabolic incorporation of chemical probes. In this review, we discuss current approaches for RNA metabolic labeling with modified ribonucleosides and their integration with Next-Generation Sequencing, mass spectrometry-based proteomics, and fluorescence microscopy in order to interrogate RNA behavior in its native context.</p> |
DOI | 10.1039/d1mo00334h |
Alternate Journal | Mol Omics |
PubMed ID | 34635895 |
PubMed Central ID | PMC8648987 |
Grant List | R01 GM132189 / GM / NIGMS NIH HHS / United States |