Interrogating the transcriptome with metabolically incorporated ribonucleosides.

TitleInterrogating the transcriptome with metabolically incorporated ribonucleosides.
Publication TypeJournal Article
Year of Publication2021
AuthorsKleiner, RE
JournalMol Omics
Volume17
Issue6
Pagination833-841
Date Published2021 Dec 06
ISSN2515-4184
Abstract

<p>RNA is a central player in biological processes, but there remain major gaps in our understanding of transcriptomic processes and the underlying biochemical mechanisms regulating RNA in cells. A powerful strategy to facilitate molecular analysis of cellular RNA is the metabolic incorporation of chemical probes. In this review, we discuss current approaches for RNA metabolic labeling with modified ribonucleosides and their integration with Next-Generation Sequencing, mass spectrometry-based proteomics, and fluorescence microscopy in order to interrogate RNA behavior in its native context.</p>

DOI10.1039/d1mo00334h
Alternate JournalMol Omics
PubMed ID34635895