Interrogating the transcriptome with metabolically incorporated ribonucleosides.

TitleInterrogating the transcriptome with metabolically incorporated ribonucleosides.
Publication TypeJournal Article
Year of Publication2021
AuthorsKleiner, RE
JournalMol Omics
Date Published2021 12 06
KeywordsHigh-Throughput Nucleotide Sequencing, Mass Spectrometry, Ribonucleosides, RNA, Transcriptome

<p>RNA is a central player in biological processes, but there remain major gaps in our understanding of transcriptomic processes and the underlying biochemical mechanisms regulating RNA in cells. A powerful strategy to facilitate molecular analysis of cellular RNA is the metabolic incorporation of chemical probes. In this review, we discuss current approaches for RNA metabolic labeling with modified ribonucleosides and their integration with Next-Generation Sequencing, mass spectrometry-based proteomics, and fluorescence microscopy in order to interrogate RNA behavior in its native context.</p>

Alternate JournalMol Omics
PubMed ID34635895
PubMed Central IDPMC8648987
Grant ListR01 GM132189 / GM / NIGMS NIH HHS / United States