The insulator functions of the polydactyl C2H2 zinc finger protein CTCF: Necessity versus sufficiency.

TitleThe insulator functions of the polydactyl C2H2 zinc finger protein CTCF: Necessity versus sufficiency.
Publication TypeJournal Article
Year of Publication2020
AuthorsKyrchanova, O, Maksimenko, O, Ibragimov, A, Sokolov, V, Postika, N, Lukyanova, M, Schedl, P, Georgiev, P
JournalSci Adv
Volume6
Issue13
Paginationeaaz3152
Date Published2020 03
ISSN2375-2548
Abstract

<p>In mammals, a C2H2 zinc finger (C2H2) protein, CTCF, acts as the master regulator of chromosomal architecture and of the expression of Hox gene clusters. Like mammalian CTCF, the homolog, dCTCF, localizes to boundaries in the bithorax complex (BX-C). Here, we have determined the minimal requirements for the assembly of a functional boundary by dCTCF and two other C2H2 zinc finger proteins, Pita and Su(Hw). Although binding sites for these proteins are essential for the insulator activity of BX-C boundaries, these binding sites alone are insufficient to create a functional boundary. dCTCF cannot effectively bind to a single recognition sequence in chromatin or generate a functional insulator without the help of additional proteins. In addition, for boundary elements in BX-C at least four binding sites for dCTCF or the presence of additional DNA binding factors is required to generate a functional insulator.</p>

DOI10.1126/sciadv.aaz3152
Alternate JournalSci Adv
PubMed ID32232161
PubMed Central IDPMC7096168
Grant ListR35 GM126975 / GM / NIGMS NIH HHS / United States