Inference of Multisite Phosphorylation Rate Constants and Their Modulation by Pathogenic Mutations.

TitleInference of Multisite Phosphorylation Rate Constants and Their Modulation by Pathogenic Mutations.
Publication TypeJournal Article
Year of Publication2020
AuthorsYeung, E, McFann, S, Marsh, L, Dufresne, E, Filippi, S, Harrington, HA, Shvartsman, SY, Wühr, M
JournalCurr Biol
Date Published2020 Mar 09
KeywordsAnimals, Cell Cycle, Humans, Mitogen-Activated Protein Kinase Kinases, Models, Biological, Mutation, Phosphorylation, Rats

<p>Multisite protein phosphorylation plays a critical role in cell regulation [1-3]. It is widely appreciated that the functional capabilities of multisite phosphorylation depend on the order and kinetics of phosphorylation steps, but kinetic aspects of multisite phosphorylation remain poorly understood [4-6]. Here, we focus on what appears to be the simplest scenario, when a protein is phosphorylated on only two sites in a strict, well-defined order. This scenario describes the activation of ERK, a highly conserved cell-signaling enzyme. We use Bayesian parameter inference in a structurally identifiable kinetic model to dissect dual phosphorylation of ERK by MEK, a kinase that is mutated in a large number of human diseases [7-12]. Our results reveal how enzyme processivity and efficiencies of individual phosphorylation steps are altered by pathogenic mutations. The presented approach, which connects specific mutations to kinetic parameters of multisite phosphorylation mechanisms, provides a systematic framework for closing the gap between studies with purified enzymes and their effects in the living organism.</p>

Alternate JournalCurr Biol
PubMed ID32059766
PubMed Central IDPMC7085240
Grant ListR01 GM086537 / GM / NIGMS NIH HHS / United States
R35 GM128813 / GM / NIGMS NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States