Induction of Diverse Cryptic Fungal Metabolites by Steroids and Channel Blockers.

TitleInduction of Diverse Cryptic Fungal Metabolites by Steroids and Channel Blockers.
Publication TypeJournal Article
Year of Publication2022
AuthorsLee, SRak, Seyedsayamdost, MR
JournalAngew Chem Int Ed Engl
Volume61
Issue29
Paginatione202204519
Date Published2022 Jul 18
ISSN1521-3773
KeywordsAgar, Anti-Bacterial Agents, Biological Products, Multigene Family, Steroids
Abstract

<p>Fungi offer a deep source of natural products but remain underutilized. Most biosynthetic gene clusters (BGCs) that can be detected are silent or "cryptic" in standard lab cultures and their products are thus not interrogated in routine screens. As genetic alterations are difficult and some strains can only be grown on agar, we have herein applied an agar-based high-throughput chemical genetic screen to identify inducers of fungal BGCs. Using R. solani and S. sclerotiorum as test cases, we report 13 cryptic metabolites in four compound groups, including sclerocyclane, a natural product with a novel scaffold. Steroids were the best elicitors and follow-up studies showed that plant-steroids trigger sclerocyclane synthesis, which shows antibiotic activity against B. plantarii, an ecological competitor of S. sclerotiorum. Our results open new paths to exploring the chemical ecology of fungal-plant interactions and provide a genetics-free approach for uncovering cryptic fungal metabolites.</p>

DOI10.1002/anie.202204519
Alternate JournalAngew Chem Int Ed Engl
PubMed ID35509119
PubMed Central IDPMC9276648
Grant ListR01 GM140034 / GM / NIGMS NIH HHS / United States