Improved Protein Splicing Using Embedded Split Inteins.

TitleImproved Protein Splicing Using Embedded Split Inteins.
Publication TypeJournal Article
Year of Publication2017
AuthorsGramespacher, JA, Stevens, AJ, Thompson, RE, Muir, TW
JournalProtein Sci
Date Published2017 Dec 11
ISSN1469-896X
Abstract

Naturally split inteins mediate a traceless protein ligation process known as protein trans-splicing (PTS). Although frequently used in protein engineering applications, the efficiency of PTS can be reduced by the tendency of some split intein fusion constructs to aggregate; a consequence of the fragmented nature of the split intein itself or the polypeptide to which it is fused (the extein). Here, we report a strategy to help address this liability. This involves embedding the split intein within a protein sequence designed to stabilize either the intein fragment itself or the appended extein. We expect this approach to increase the scope of PTS-based protein engineering efforts. This article is protected by copyright. All rights reserved.

DOI10.1002/pro.3357
Alternate JournalProtein Sci.
PubMed ID29226478