Title | Improved protein splicing using embedded split inteins. |
Publication Type | Journal Article |
Year of Publication | 2018 |
Authors | Gramespacher, JA, Stevens, AJ, Thompson, RE, Muir, TW |
Journal | Protein Sci |
Volume | 27 |
Issue | 3 |
Pagination | 614-619 |
Date Published | 2018 Mar |
ISSN | 1469-896X |
Keywords | Amino Acid Sequence, Inteins, Protein Engineering, Protein Splicing, Proteins |
Abstract | <p>Naturally split inteins mediate a traceless protein ligation process known as protein trans-splicing (PTS). Although frequently used in protein engineering applications, the efficiency of PTS can be reduced by the tendency of some split intein fusion constructs to aggregate; a consequence of the fragmented nature of the split intein itself or the polypeptide to which it is fused (the extein). Here, we report a strategy to help address this liability. This involves embedding the split intein within a protein sequence designed to stabilize either the intein fragment itself or the appended extein. We expect this approach to increase the scope of PTS-based protein engineering efforts.</p> |
DOI | 10.1002/pro.3357 |
Alternate Journal | Protein Sci |
PubMed ID | 29226478 |
PubMed Central ID | PMC5818749 |
Grant List | R37 GM086868 / GM / NIGMS NIH HHS / United States T32 GM007388 / GM / NIGMS NIH HHS / United States |