|Title||Improved Protein Splicing Using Embedded Split Inteins.|
|Publication Type||Journal Article|
|Year of Publication||2017|
|Authors||Gramespacher, JA, Stevens, AJ, Thompson, RE, Muir, TW|
|Date Published||2017 Dec 11|
Naturally split inteins mediate a traceless protein ligation process known as protein trans-splicing (PTS). Although frequently used in protein engineering applications, the efficiency of PTS can be reduced by the tendency of some split intein fusion constructs to aggregate; a consequence of the fragmented nature of the split intein itself or the polypeptide to which it is fused (the extein). Here, we report a strategy to help address this liability. This involves embedding the split intein within a protein sequence designed to stabilize either the intein fragment itself or the appended extein. We expect this approach to increase the scope of PTS-based protein engineering efforts. This article is protected by copyright. All rights reserved.
|Alternate Journal||Protein Sci.|