Improved protein splicing using embedded split inteins.

TitleImproved protein splicing using embedded split inteins.
Publication TypeJournal Article
Year of Publication2018
AuthorsGramespacher, JA, Stevens, AJ, Thompson, RE, Muir, TW
JournalProtein Sci
Volume27
Issue3
Pagination614-619
Date Published2018 03
ISSN1469-896X
KeywordsAmino Acid Sequence, Inteins, Protein Engineering, Protein Splicing, Proteins
Abstract

<p>Naturally split inteins mediate a traceless protein ligation process known as protein trans-splicing (PTS). Although frequently used in protein engineering applications, the efficiency of PTS can be reduced by the tendency of some split intein fusion constructs to aggregate; a consequence of the fragmented nature of the split intein itself or the polypeptide to which it is fused (the extein). Here, we report a strategy to help address this liability. This involves embedding the split intein within a protein sequence designed to stabilize either the intein fragment itself or the appended extein. We expect this approach to increase the scope of PTS-based protein engineering efforts.</p>

DOI10.1002/pro.3357
Alternate JournalProtein Sci
PubMed ID29226478
PubMed Central IDPMC5818749
Grant ListR37 GM086868 / GM / NIGMS NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States