Immunization with AgTRIO, a Protein in Anopheles Saliva, Contributes to Protection against Plasmodium Infection in Mice.

TitleImmunization with AgTRIO, a Protein in Anopheles Saliva, Contributes to Protection against Plasmodium Infection in Mice.
Publication TypeJournal Article
Year of Publication2018
AuthorsDragovic, SM, Agunbiade, TA, Freudzon, M, Yang, J, Hastings, AK, Schleicher, TR, Zhou, X, Craft, S, Chuang, Y-M, Gonzalez, F, Li, Y, Hrebikova, G, Tripathi, A, Mlambo, G, Almeras, L, Ploss, A, Dimopoulos, G, Fikrig, E
JournalCell Host Microbe
Volume23
Issue4
Pagination523-535.e5
Date Published2018 04 11
ISSN1934-6069
KeywordsAnimals, Anopheles, Disease Models, Animal, Immunization, Passive, Insect Proteins, Liver, Malaria, Mice, Parasite Load, Parasitemia, Plasmodium berghei, Plasmodium falciparum, Salivary Proteins and Peptides, Treatment Outcome
Abstract

<p>Plasmodium infection begins with the bite of an anopheline mosquito, when sporozoites along with saliva are injected into a vertebrate host. The role of the host responses to mosquito saliva components in malaria remains unclear. We observed that antisera against Anopheles gambiae salivary glands partially protected mice from mosquito-borne Plasmodium infection. Specifically, antibodies to A. gambiae TRIO (AgTRIO), a mosquito salivary gland antigen, contributed to the protection. Mice administered AgTRIO antiserum showed lower Plasmodium liver burden and decreased parasitemia when exposed to infected mosquitoes. Active immunization with AgTRIO was also partially protective against Plasmodium berghei infection. A combination of AgTRIO antiserum and antibodies against Plasmodium circumsporozoite protein, a vaccine candidate, further decreased P. berghei infection. In humanized mice, AgTRIO antiserum afforded some protection against mosquito-transmitted Plasmodium falciparum. AgTRIO antiserum reduced the movement of sporozoites in the murine dermis. AgTRIO may serve as an arthropod-based target against Plasmodium to combat malaria.</p>

DOI10.1016/j.chom.2018.03.008
Alternate JournalCell Host Microbe
PubMed ID29649443
PubMed Central IDPMC5998332
Grant ListR21 AI131574 / AI / NIAID NIH HHS / United States
T32 AR007016 / AR / NIAMS NIH HHS / United States
R01 GM054787 / GM / NIGMS NIH HHS / United States
R01 AI129862 / AI / NIAID NIH HHS / United States