Illuminating developmental biology with cellular optogenetics.

TitleIlluminating developmental biology with cellular optogenetics.
Publication TypeJournal Article
Year of Publication2018
AuthorsJohnson, HE, Toettcher, JE
JournalCurr Opin Biotechnol
Volume52
Pagination42-48
Date Published2018 Mar 02
ISSN1879-0429
Abstract

In developmental biology, localization is everything. The same stimulus-cell signaling event or expression of a gene-can have dramatically different effects depending on the time, spatial position, and cell types in which it is applied. Yet the field has long lacked the ability to deliver localized perturbations with high specificity in vivo. The advent of optogenetic tools, capable of delivering highly localized stimuli, is thus poised to profoundly expand our understanding of development. We describe the current state-of-the-art in cellular optogenetic tools, review the first wave of major studies showcasing their application in vivo, and discuss major obstacles that must be overcome if the promise of developmental optogenetics is to be fully realized.

DOI10.1016/j.copbio.2018.02.003
Alternate JournalCurr. Opin. Biotechnol.
PubMed ID29505976
Grant ListDP2 EB024247 / EB / NIBIB NIH HHS / United States
F32 GM119297 / GM / NIGMS NIH HHS / United States