Title | Identification of a Molecular Latch that Regulates Staphylococcal Virulence. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Xie, Q, Zhao, A, Jeffrey, PD, Kim, MKevin, Bassler, BL, Stone, HA, Novick, RP, Muir, TW |
Journal | Cell Chem Biol |
Volume | 26 |
Issue | 4 |
Pagination | 548-558.e4 |
Date Published | 2019 04 18 |
ISSN | 2451-9448 |
Keywords | Allosteric Regulation, Bacterial Proteins, Humans, Hydrogen Bonding, Molecular Docking Simulation, Peptides, Cyclic, Protein Conformation, Protein Kinases, Quorum Sensing, Signal Transduction, Staphylococcal Infections, Staphylococcus aureus, Virulence |
Abstract | <p>Virulence induction in the Staphylococcus aureus is under the control of a quorum sensing (QS) circuit encoded by the accessory gene regulator (agr) locus. Allelic variation within agr produces four QS specificity groups, each producing a unique secreted autoinducer peptide (AIP) and receptor histidine kinase (RHK), AgrC. Cognate AIP-AgrC interactions activate virulence through a two-component signaling cascade, whereas non-cognate pairs are generally inhibitory. Here we pinpoint a key hydrogen-bonding interaction within AgrC that acts as a switch to convert helical motions propagating from the receptor sensor domain into changes in inter-domain association within the kinase module. AgrC mutants lacking this interaction are constitutively active in vitro and in vivo, the latter leading to a pronounced attenuation of S. aureus biofilm formation. Thus, our work sheds light on the regulation of this biomedically important RHK.</p> |
DOI | 10.1016/j.chembiol.2019.01.006 |
Alternate Journal | Cell Chem Biol |
PubMed ID | 30773482 |
PubMed Central ID | PMC6506218 |
Grant List | / HHMI / Howard Hughes Medical Institute / United States P41 GM103485 / GM / NIGMS NIH HHS / United States R01 AI042783 / AI / NIAID NIH HHS / United States R01 GM095880 / GM / NIGMS NIH HHS / United States |