How Microtubules Build the Spindle Branch by Branch.

TitleHow Microtubules Build the Spindle Branch by Branch.
Publication TypeJournal Article
Year of Publication2022
AuthorsTravis, SM, Mahon, BP, Petry, S
JournalAnnu Rev Cell Dev Biol
Date Published2022/06/27
ISSN1530-8995
Abstract

The microtubule (MT) cytoskeleton provides the architecture that governs intracellular organization and the regulated motion of macromolecules through the crowded cytoplasm. The key to establishing a functioning cytoskeletal architecture is regulating when and where new MTs are nucleated. Within the spindle, the vast majority of MTs are generated through a pathway known as branching MT nucleation, which exponentially amplifies MT number in a polar manner. Whereas other MT nucleation pathways generally require a complex organelle such as the centrosome or Golgi apparatus to localize nucleation factors, the branching site is based solely on a simple, preformed MT, making it an ideal system to study MT nucleation. In this review, we address recent developments in characterizing branching factors, the branching reaction, and its regulation, as well as branching MT nucleation in systems beyond the spindle and within human disease. Expected final online publication date for the Volume 38 is October 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

DOI10.1146/annurev-cellbio-120420-114559
Alternate JournalAnnu Rev Cell Dev Biol
PubMed ID35759800