A Host-Produced Quorum-Sensing Autoinducer Controls a Phage Lysis-Lysogeny Decision.

TitleA Host-Produced Quorum-Sensing Autoinducer Controls a Phage Lysis-Lysogeny Decision.
Publication TypeJournal Article
Year of Publication2019
AuthorsSilpe, JE, Bassler, BL
JournalCell
Volume176
Issue1-2
Pagination268-280.e13
Date Published2019 01 10
ISSN1097-4172
KeywordsBacteriophages, Biofilms, DNA-Binding Proteins, Gene Expression Regulation, Bacterial, Lysogeny, Pyrazoles, Quorum Sensing, Vibrio, Vibrio cholerae, Virulence, Virus Latency
Abstract

Vibrio cholerae uses a quorum-sensing (QS) system composed of the autoinducer 3,5-dimethylpyrazin-2-ol (DPO) and receptor VqmA (VqmAVc), which together repress genes for virulence and biofilm formation. vqmA genes exist in Vibrio and in one vibriophage, VP882. Phage-encoded VqmA (VqmAPhage) binds to host-produced DPO, launching the phage lysis program via an antirepressor that inactivates the phage repressor by sequestration. The antirepressor interferes with repressors from related phages. Like phage VP882, these phages encode DNA-binding proteins and partner antirepressors, suggesting that they, too, integrate host-derived information into their lysis-lysogeny decisions. VqmAPhage activates the host VqmAVc regulon, whereas VqmAVc cannot induce phage-mediated lysis, suggesting an asymmetry whereby the phage influences host QS while enacting its own lytic-lysogeny program without interference. We reprogram phages to activate lysis in response to user-defined cues. Our work shows that a phage, causing bacterial infections, and V. cholerae, causing human infections, rely on the same signal molecule for pathogenesis.

DOI10.1016/j.cell.2018.10.059
Alternate JournalCell
PubMed ID30554875
PubMed Central IDPMC6329655
Grant List / / Howard Hughes Medical Institute / United States
R01 GM065859 / GM / NIGMS NIH HHS / United States
R37 GM065859 / GM / NIGMS NIH HHS / United States