Title | A Host-Produced Autoinducer-2 Mimic Activates Bacterial Quorum Sensing. |
Publication Type | Journal Article |
Year of Publication | 2016 |
Authors | Ismail, AS, Valastyan, JS, Bassler, BL |
Journal | Cell Host Microbe |
Volume | 19 |
Issue | 4 |
Pagination | 470-80 |
Date Published | 2016 Apr 13 |
ISSN | 1934-6069 |
Keywords | Animals, Bacterial Proteins, Epithelial Cells, Gene Expression Regulation, Bacterial, Homoserine, Host-Pathogen Interactions, Humans, Lactones, Quorum Sensing, Salmonella Infections, Salmonella typhimurium |
Abstract | <p>Host-microbial symbioses are vital to health; nonetheless, little is known about the role crosskingdom signaling plays in these relationships. In a process called quorum sensing, bacteria communicate with one another using extracellular signal molecules called autoinducers. One autoinducer, AI-2, is proposed to promote interspecies bacterial communication, including in the mammalian gut. We show that mammalian epithelia produce an AI-2 mimic activity in response to bacteria or tight-junction disruption. This AI-2 mimic is detected by the bacterial AI-2 receptor, LuxP/LsrB, and can activate quorum-sensing-controlled gene expression, including in the enteric pathogen Salmonella typhimurium. AI-2 mimic activity is induced when epithelia are directly or indirectly exposed to bacteria, suggesting that a secreted bacterial component(s) stimulates its production. Mutagenesis revealed genes required for bacteria to both detect and stimulate production of the AI-2 mimic. These findings uncover a potential role for the mammalian AI-2 mimic in fostering crosskingdom signaling and host-bacterial symbioses.</p> |
DOI | 10.1016/j.chom.2016.02.020 |
Alternate Journal | Cell Host Microbe |
PubMed ID | 26996306 |
PubMed Central ID | PMC4869860 |
Grant List | R01 GM065859 / GM / NIGMS NIH HHS / United States F32 GM100711 / GM / NIGMS NIH HHS / United States 5F32GM100711-02 / GM / NIGMS NIH HHS / United States / / Howard Hughes Medical Institute / United States R37 GM065859 / GM / NIGMS NIH HHS / United States 5R01GM065859 / GM / NIGMS NIH HHS / United States |