Homologous organization of cerebellar pathways to sensory, motor, and associative forebrain.

TitleHomologous organization of cerebellar pathways to sensory, motor, and associative forebrain.
Publication TypeJournal Article
Year of Publication2021
AuthorsPisano, TJ, Dhanerawala, ZM, Kislin, M, Bakshinskaya, D, Engel, EA, Hansen, EJ, Hoag, AT, Lee, J, de Oude, NL, Venkataraju, KUmadevi, Verpeut, JL, Hoebeek, FE, Richardson, BD, Boele, H-J, Wang, SS-H
JournalCell Rep
Volume36
Issue12
Pagination109721
Date Published2021 Sep 21
ISSN2211-1247
KeywordsAnimals, Cerebellum, Cerebral Cortex, Female, Genetic Vectors, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, Neural Pathways, Proto-Oncogene Proteins c-fos, Simplexvirus, Thalamic Nuclei
Abstract

<p>Cerebellar outputs take polysynaptic routes to reach the rest of the brain, impeding conventional tracing. Here, we quantify pathways between the cerebellum and forebrain by using transsynaptic tracing viruses and a whole-brain analysis pipeline. With retrograde tracing, we find that most descending paths originate from the somatomotor cortex. Anterograde tracing of ascending paths encompasses most thalamic nuclei, especially ventral posteromedial, lateral posterior, mediodorsal, and reticular nuclei. In the neocortex, sensorimotor regions contain the most labeled neurons, but we find higher densities in associative areas, including orbital, anterior cingulate, prelimbic, and infralimbic cortex. Patterns of ascending expression correlate with c-Fos expression after optogenetic inhibition of Purkinje cells. Our results reveal homologous networks linking single areas of the cerebellar cortex to diverse forebrain targets. We conclude that shared areas of the cerebellum are positioned to provide sensory-motor information to regions implicated in both movement and nonmotor function.</p>

DOI10.1016/j.celrep.2021.109721
Alternate JournalCell Rep
PubMed ID34551311
PubMed Central IDPMC8506234
Grant ListF31 NS089303 / NS / NINDS NIH HHS / United States
P40 OD010996 / OD / NIH HHS / United States
R01 MH115750 / MH / NIMH NIH HHS / United States
P20 GM103408 / GM / NIGMS NIH HHS / United States
U19 NS104648 / NS / NINDS NIH HHS / United States
R21 DC018365 / DC / NIDCD NIH HHS / United States
R01 NS045193 / NS / NINDS NIH HHS / United States