Title | High-throughput approaches to profile RNA-protein interactions. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Nechay, M, Kleiner, RE |
Journal | Curr Opin Chem Biol |
Volume | 54 |
Pagination | 37-44 |
Date Published | 2020 Feb |
ISSN | 1879-0402 |
Keywords | Animals, Binding Sites, Gene Expression Profiling, High-Throughput Screening Assays, Humans, Mass Spectrometry, Proteins, Proteomics, RNA |
Abstract | <p>RNA-protein interactions play a critical role in post-transcriptional gene regulation. Characterizing these interactions in their native context has been challenging; however, advances in RNA sequencing and mass spectrometrybased proteomics combined with innovative chemical biological tools have heralded the development of robust strategies for performing biochemistry on a cellular scale. Herein, we review recent advances in the development and application of proteomic and transcriptomic approaches to profile cellular RNA-protein interactions, focusing on sequencing-based strategies and proteomic analysis of RNA-binding proteins, as well as approaches to address the role of RNA modifications in protein-RNA binding events.</p> |
DOI | 10.1016/j.cbpa.2019.11.002 |
Alternate Journal | Curr Opin Chem Biol |
PubMed ID | 31812895 |
PubMed Central ID | PMC7131871 |
Grant List | R01 GM132189 / GM / NIGMS NIH HHS / United States |