High-throughput approaches to profile RNA-protein interactions. Author Misha Nechay, Ralph Kleiner Publication Year 2020 Type Journal Article Abstract RNA-protein interactions play a critical role in post-transcriptional gene regulation. Characterizing these interactions in their native context has been challenging; however, advances in RNA sequencing and mass spectrometrybased proteomics combined with innovative chemical biological tools have heralded the development of robust strategies for performing biochemistry on a cellular scale. Herein, we review recent advances in the development and application of proteomic and transcriptomic approaches to profile cellular RNA-protein interactions, focusing on sequencing-based strategies and proteomic analysis of RNA-binding proteins, as well as approaches to address the role of RNA modifications in protein-RNA binding events. Keywords Animals, Gene Expression Profiling, Humans, Binding Sites, High-Throughput Screening Assays, RNA, Mass Spectrometry, Proteomics, Proteins Journal Curr Opin Chem Biol Volume 54 Pages 37-44 Date Published 2020 Feb ISSN Number 1879-0402 DOI 10.1016/j.cbpa.2019.11.002 Alternate Journal Curr Opin Chem Biol PMCID PMC7131871 PMID 31812895 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML