High-throughput approaches to profile RNA-protein interactions.

TitleHigh-throughput approaches to profile RNA-protein interactions.
Publication TypeJournal Article
Year of Publication2020
AuthorsNechay, M, Kleiner, RE
JournalCurr Opin Chem Biol
Date Published2020 Feb
KeywordsAnimals, Binding Sites, Gene Expression Profiling, High-Throughput Screening Assays, Humans, Mass Spectrometry, Proteins, Proteomics, RNA

<p>RNA-protein interactions play a critical role in post-transcriptional gene regulation. Characterizing these interactions in their native context has been challenging; however, advances in RNA sequencing and mass spectrometrybased proteomics combined with innovative chemical biological tools have heralded the development of robust strategies for performing biochemistry on a cellular scale. Herein, we review recent advances in the development and application of proteomic and transcriptomic approaches to profile cellular RNA-protein interactions, focusing on sequencing-based strategies and proteomic analysis of RNA-binding proteins, as well as approaches to address the role of RNA modifications in protein-RNA binding events.</p>

Alternate JournalCurr Opin Chem Biol
PubMed ID31812895
PubMed Central IDPMC7131871
Grant ListR01 GM132189 / GM / NIGMS NIH HHS / United States