Global morphogenetic flow is accurately predicted by the spatial distribution of myosin motors.

TitleGlobal morphogenetic flow is accurately predicted by the spatial distribution of myosin motors.
Publication TypeJournal Article
Year of Publication2018
AuthorsStreichan, SJ, Lefebvre, MF, Noll, N, Wieschaus, EF, Shraiman, BI
JournalElife
Volume7
Date Published2018 02 09
ISSN2050-084X
KeywordsAnimals, Drosophila, Gastrulation, Microscopy, Myosins, Spatio-Temporal Analysis
Abstract

<p>During embryogenesis tissue layers undergo morphogenetic flow rearranging and folding into specific shapes. While developmental biology has identified key genes and local cellular processes, global coordination of tissue remodeling at the organ scale remains unclear. Here, we combine light-sheet microscopy of the embryo with quantitative analysis and physical modeling to relate cellular flow with the patterns of force generation during the gastrulation process. We find that the complex spatio-temporal flow pattern can be predicted from the measured meso-scale myosin density and anisotropy using a simple, effective viscous model of the tissue, achieving close to 90% accuracy with one time dependent and two constant parameters. Our analysis uncovers the importance of a) spatial modulation of myosin distribution on the scale of the embryo and b) the non-locality of its effect due to mechanical interaction of cells, demonstrating the need for the global perspective in the study of morphogenetic flow.</p>

DOI10.7554/eLife.27454
Alternate JournalElife
PubMed ID29424685
PubMed Central IDPMC5843464
Grant ListK99 HD088708 / HD / NICHD NIH HHS / United States
R00 HD088708 / HD / NICHD NIH HHS / United States
NICHD 1K99HD088708 / HD / NICHD NIH HHS / United States
/ HHMI / Howard Hughes Medical Institute / United States