Germ Cell-less Promotes Centrosome Segregation to Induce Germ Cell Formation.

TitleGerm Cell-less Promotes Centrosome Segregation to Induce Germ Cell Formation.
Publication TypeJournal Article
Year of Publication2017
AuthorsLerit, DA, Shebelut, CW, Lawlor, KJ, Rusan, NM, Gavis, ER, Schedl, P, Deshpande, G
JournalCell Rep
Date Published2017 01 24
KeywordsAnimals, Centrosome, Chromosome Segregation, Drosophila, Drosophila Proteins, Embryo, Nonmammalian, Germ Cells, Intercellular Signaling Peptides and Proteins, Kinesins, Microscopy, Fluorescence, Nuclear Proteins, RNA Interference, RNA, Small Interfering, Time-Lapse Imaging, Tubulin

<p>The primordial germ cells (PGCs) specified during embryogenesis serve as progenitors to the adult germline stem cells. In Drosophila, the proper specification and formation of PGCs require both centrosomes and germ plasm, which contains the germline determinants. Centrosomes are microtubule (MT)-organizing centers that ensure the faithful segregation of germ plasm into PGCs. To date, mechanisms that modulate centrosome behavior to engineer PGC development have remained elusive. Only one germ plasm component, Germ cell-less (Gcl), is known to play a role in PGC formation. Here, we show that Gcl engineers PGC formation by regulating centrosome dynamics. Loss of gcl leads to aberrant centrosome separation and elaboration of the astral MT network, resulting in inefficient germ plasm segregation and aborted PGC cellularization. Importantly, compromising centrosome separation alone is sufficient to mimic the gcl loss-of-function phenotypes. We conclude Gcl functions as a key regulator of centrosome separation required for proper PGC development.</p>

Alternate JournalCell Rep
PubMed ID28122234
PubMed Central IDPMC5327791
Grant ListR01 GM067758 / GM / NIGMS NIH HHS / United States
K22 HL126922 / HL / NHLBI NIH HHS / United States
R01 GM110015 / GM / NIGMS NIH HHS / United States
ZIA HL006126 / / Intramural NIH HHS / United States
ZIA HL006126-06 / / Intramural NIH HHS / United States