Title | Germ Cell-less Promotes Centrosome Segregation to Induce Germ Cell Formation. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Lerit, DA, Shebelut, CW, Lawlor, KJ, Rusan, NM, Gavis, ER, Schedl, P, Deshpande, G |
Journal | Cell Rep |
Volume | 18 |
Issue | 4 |
Pagination | 831-839 |
Date Published | 2017 01 24 |
ISSN | 2211-1247 |
Keywords | Animals, Centrosome, Chromosome Segregation, Drosophila, Drosophila Proteins, Embryo, Nonmammalian, Germ Cells, Intercellular Signaling Peptides and Proteins, Kinesins, Microscopy, Fluorescence, Nuclear Proteins, RNA Interference, RNA, Small Interfering, Time-Lapse Imaging, Tubulin |
Abstract | <p>The primordial germ cells (PGCs) specified during embryogenesis serve as progenitors to the adult germline stem cells. In Drosophila, the proper specification and formation of PGCs require both centrosomes and germ plasm, which contains the germline determinants. Centrosomes are microtubule (MT)-organizing centers that ensure the faithful segregation of germ plasm into PGCs. To date, mechanisms that modulate centrosome behavior to engineer PGC development have remained elusive. Only one germ plasm component, Germ cell-less (Gcl), is known to play a role in PGC formation. Here, we show that Gcl engineers PGC formation by regulating centrosome dynamics. Loss of gcl leads to aberrant centrosome separation and elaboration of the astral MT network, resulting in inefficient germ plasm segregation and aborted PGC cellularization. Importantly, compromising centrosome separation alone is sufficient to mimic the gcl loss-of-function phenotypes. We conclude Gcl functions as a key regulator of centrosome separation required for proper PGC development.</p> |
DOI | 10.1016/j.celrep.2016.12.074 |
Alternate Journal | Cell Rep |
PubMed ID | 28122234 |
PubMed Central ID | PMC5327791 |
Grant List | R01 GM067758 / GM / NIGMS NIH HHS / United States K22 HL126922 / HL / NHLBI NIH HHS / United States R01 GM110015 / GM / NIGMS NIH HHS / United States ZIA HL006126 / / Intramural NIH HHS / United States ZIA HL006126-06 / / Intramural NIH HHS / United States |