Genetic Analysis of Protein Translocation.

TitleGenetic Analysis of Protein Translocation.
Publication TypeJournal Article
Year of Publication2019
AuthorsSilhavy, TJ, Mitchell, AM
JournalProtein J
Volume38
Issue3
Pagination217-228
Date Published2019 06
ISSN1875-8355
KeywordsArtificial Gene Fusion, beta-Galactosidase, Cell Membrane, Escherichia coli, Escherichia coli Proteins, Gene Fusion, Lac Operon, Protein Transport, Recombinant Fusion Proteins, SEC Translocation Channels
Abstract

<p>Cells in all domains of life must translocate newly synthesized proteins both across membranes and into membranes. In eukaryotes, proteins are translocated into the lumen of the ER or the ER membrane. In prokaryotes, proteins are translocated into the cytoplasmic membrane or through the membrane into the periplasm for Gram-negative bacteria or the extracellular space for Gram-positive bacteria. Much of what we know about protein translocation was learned through genetic selections and screens utilizing lacZ gene fusions in Escherichia coli. This review covers the basic principles of protein translocation and how they were discovered and developed. In particular, we discuss how lacZ gene fusions and the phenotypes conferred were exploited to identify the genes involved in protein translocation and provide insights into their mechanisms of action. These approaches, which allowed the elucidation of processes that are conserved throughout the domains of life, illustrate the power of seemingly simple experiments.</p>

DOI10.1007/s10930-019-09813-y
Alternate JournalProtein J
PubMed ID30684070
PubMed Central IDPMC6589372
Grant ListR35 GM118024 / GM / NIGMS NIH HHS / United States
R35GM118024 / GM / NIGMS NIH HHS / United States